Recombinant Human GCDH, His-tagged

• Cat.No.: GCDH-27464TH
• Product Overview: Recombinant full length Human GCDH , with N terminal His-tag; 415aa, 45.8kDa inclusive of tag

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 394 amino acids
• Description: The protein encoded by this gene belongs to the acyl-CoA dehydrogenase family. It catalyzes the oxidative decarboxylation of glutaryl-CoA to crotonyl-CoA and CO(2) in the degradative pathway of L-lysine, L-hydroxylysine, and L-tryptophan metabolism. It uses electron transfer flavoprotein as its electron acceptor. The enzyme exists in the mitochondrial matrix as a homotetramer of 45-kD subunits. Alternatively spliced transcript variants encoding different isoforms have been identified.
• Conjugation: HIS
• Molecular Weight: 45.800kDa inclusive of tags
• Tissue specificity: Isoform 1 and isoform 2 are expressed in fibroblasts and liver.
• Form: Liquid
• Purity: >90% by SDS-PAGE
• Storage buffer: Preservative: NoneConstituents: 20% Glycerol, 5mM DTT, 200mM Sodium chloride, 20mM Tris HCl, pH 8
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Sequences of amino acids: MGSSHHHHHHSSGLVPRGSHMRPEFDWQDPLVLEEQLTTDEILIRDTFRTYCQERLMPRILLANRNEVFHREIISEMGELGVLGPTIKGYGCAGVSSVAYGLLARELERVDSGYRSAMSVQSSLVMHPIYAYGSEEQRQKYLPQLAKGELLGCFGLTEPNSGSDPSSMETRAHYNSSNKSYTLNGTKTWITNSPMADLFVVWARCEDGCIRGFLLEKGMRGLSAPRIQGKFSLRASATGMIIMDGVEVPEENVLPGASSLGGPFGCLNNARYGIAWGVLGASEFCLHTARQYALDRMQFGVPLARNQLIQKKLADMLTEITLGLHACLQLGRLKDQDKAAPEMVSLLKRNNCGKALDIARQARDMLGGNGISDEYHVIRHAMNLEAVNTYEGTHDIHALILGRAITGIQAFTASK
• Sequence Similarities: Belongs to the acyl-CoA dehydrogenase family.

Gene Information

• Gene Name: GCDH glutaryl-CoA dehydrogenase [ Homo sapiens ]
• Official Symbol: GCDH
• Synonyms: GCDH; glutaryl-CoA dehydrogenase; glutaryl Coenzyme A dehydrogenase; glutaryl-CoA dehydrogenase, mitochondrial; ACAD5
• Gene ID: 2639
• mRNA Refseq: NM_000159
• Protein Refseq: NP_000150
• MIM: 608801
• Uniprot ID: Q92947
• Chromosome Location: 19p13.2
• Pathway: Fatty Acid Beta Oxidation, organism-specific biosystem; Fatty acid metabolism, organism-specific biosystem; Fatty acid metabolism, conserved biosystem; Lysine catabolism, organism-specific biosystem; Lysine degradation, organism-specific biosystem
• Function: fatty-acyl-CoA binding; flavin adenine dinucleotide binding; glutaryl-CoA dehydrogenase activity