Recombinant Human GCLC Protein, GST-tagged

• Cat.No.: GCLC-4800H
• Product Overview: Human GCLC full-length ORF ( NP_001489.1, 1 a.a. - 637 a.a.) recombinant protein with GST-tag at N-terminal.

Specification

• Description: Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. The gene encoding the catalytic subunit encodes a protein of 367 amino acids with a calculated molecular weight of 72.773 kDa and maps to chromosome 6. The regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Deficiency of gamma-glutamylcysteine synthetase in human is associated with enzymopathic hemolytic anemia. [provided by RefSeq
• Source: Wheat Germ
• Species: Human
• Tag: GST
• Molecular Mass: 99.2 kDa
• AA Sequence: MGLLSQGSPLSWEETKRHADHVRRH GILQFLHIYHAVKDRHKDVLKWGDE VEYMLVSFDHENKKVRLVLSGEKVL ETLQEKGERTNPNHPTLWRPEYGSY MIEGTPGQPYGGTMSEFNTVEANMR KRRKEATSILEENQALCTITSFPRL GCPGFTLPEVKPNPVEGGASKSLFF PDEAINKHPRFSTLTRNIRHRRGEK VVINVPIFKDKNTPSPFIETFTEDD EASRASKPDHIYMDAMGFGMGNCCL QVTFQACSISEARYLYDQLATICPI VMALSAASPFYRGYVSDIDCRWGVI SASVDDRTREERGLEPLKNNNYRIS KSRYDSIDSYLSKCGEKYNDIDLTI DKEIYEQLLQEGIDHLLAQHVAHLF IRDPLTLFEEKIHLDDANESDHFEN IQSTNWQTMRFKPPPPNSDIGWRVE FRPMEVQLTDFENSAYVVFVVLLTR VILSYKLDFLIPLSKVDENMKVAQK RDAVLQGMFYFRKDICKGGNAVVDG CGKAQNSTELAAEEYTLMSIDTIIN GKEGVFPGLIPILNSYLENMEVDVD TRCSILNYLKLIKKRASGELMTVAR WMREFIANHPDYKQDSVITDEMNYS LILKCNQIANELCECPELLGSAFRK VKYSGSKTDSSN
• Applications: Enzyme-linked Immunoabsorbent Assay
  Western Blot (Recombinant protein)
  Antibody Production
  Protein Array
• Notes: Best use within three months from the date of receipt of this protein.
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.

Gene Information

• Gene Name: GCLC glutamate-cysteine ligase, catalytic subunit [ Homo sapiens ]
• Official Symbol: GCLC
• Synonyms: GCLC; glutamate-cysteine ligase, catalytic subunit; GLCL, GLCLC; glutamate--cysteine ligase catalytic subunit; GCS; gamma-ECS; GCS heavy chain; gamma-glutamylcysteine synthetase; GCL; GLCL; GLCLC
• Gene ID: 2729
• mRNA Refseq: NM_001197115
• Protein Refseq: NP_001184044
• MIM: 606857
• UniProt ID: P48506