| Description : |
Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate-limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. This locus encodes the catalytic subunit, while the regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Mutations at this locus have been associated with hemolytic anemia due to deficiency of gamma-glutamylcysteine synthetase and susceptibility to myocardial infarction. |
| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
Myc/DDK |
| Molecular Mass : |
72.8 kDa |
| AA Sequence : |
MGLLSQGSPLSWEETKRHADHVRRH GILQFLHIYHAVKDRHKDVLKWGDE VEYMLVSFDHENKKVRLVLSGEKVL ETLQEKGERTNPNHPTLWRPEYGSY MIEGTPGQPYGGTMSEFNTVEANMR KRRKEATSILEENQALCTITSFPRL GCPGFTLPEVKPNPVEGGASKSLFF PDEAINKHPRFSTLTRNIRHRRGEK VVINVPIFKDKNTPSPFIETFTEDD EASRASKPDHIYMDAMGFGMGNCCL QVTFQACSISEARYLYDQLATICPI VMALSAASPFYRGYVSDIDCRWGVI SASVDDRTREERGLEPLKNNNYRIS KSRYDSIDSYLSKCGEKYNDIDLTI DKEIYEQLLQEGIDHLLAQHVAHLF IRDPLTLFEEKIHLDDANESDHFEN IQSTNWQTMRFKPPPPNSDIGWRVE FRPMEVQLTDFENSAYVVFVVLLTR VILSYKLDFLIPLSKVDENMKVAQK RDAVLQGMFYFRKDICKGGNAVVDG CGKAQNSTELAAEEYTLMSIDTIIN GKEGVFPGLIPILNSYLENMEVDVD TRCSILNYLKLIKKRASGELMTVAR WMREFIANHPDYKQDSVITDEMNYS LILKCNQIANELCECPELLGSAFRK VKYSGSKTDSSNTRTRPLEQKLISE EDLAANDILDYKDDDDKV |
| Purity : |
> 80% as determined by SDS-PAGE and Coomassie blue staining |
| Stability : |
Stable for 3 months from receipt of products under proper storage and handling conditions. |
| Storage : |
Store at -80 centigrade. Avoid repeated freeze-thaw cycles. |
| Concentration : |
50 μg/mL as determined by BCA |
| Storage Buffer : |
100 mM glycine, 25 mM Tris-HCl, pH 7.3. |
