Active Recombinant Human GDF15 protein(H202D), hFc-tagged

• Cat.No.: GDF15-6743H
• Product Overview: Active Recombinant Human GDF15 protein(Q99988)(Ala197-Ile308 (H202D)) is expressed from HEK293 with hFc (IgG1) tag at the N-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: 197-308 aa(H202D)
• Form: Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4).
• Bio-activity: Immobilized Human GFRAL, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human GDF15 (H202D), hFc Tag with the EC50 of 4.1ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 37.88 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 1 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
• Storage: Store at -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.
• protein Refseq-Weblink: http://www.ncbi.nlm.nih.gov/protein/NP_004855.2
• Unit ID: Q99988
• Unit ID-Weblink: http://www.uniprot.org/uniprot/Q99988

Gene Information

• Gene Name: GDF15 growth differentiation factor 15 [ Homo sapiens ]
• Official Symbol: GDF15
• Synonyms: GDF15; growth differentiation factor 15; growth/differentiation factor 15; MIC 1; MIC1; NAG 1; PDF; PLAB; prostate differentiation factor; PTGFB; NRG-1; PTGF-beta; placental TGF-beta; NSAID-activated gene 1 protein; NSAID-regulated gene 1 protein; macrophage inhibitory cytokine 1; placental bone morphogenetic protein; NSAID (nonsteroidal anti-inflammatory drug)-activated protein 1; MIC-1; NAG-1; GDF-15
• Gene ID: 9518
• mRNA Refseq: NM_004864
• protein Refseq: NP_004855
• MIM: 605312