Recombinant Human GFAP protein, GST-tagged
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|Product Overview :||Recombinant Human GFAP Protein(NP_001124491.1)(83-432 aa) is expressed from E. coli with a GST Tag.|
|Description :||This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms.|
|Source :||E. coli|
|Form :||The purified protein was Lyophilized from sterile PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH7.4), 5% trehalose and 5% mannitol are added as protectants before lyophilization.|
|Bio-activity :||Not tested|
|Protein length :||83-432 aa|
|AA Sequence :||YIEKVRFLEQQNKAL AAELNQLRAKEPTKL ADVYQAELRELRLRL DQLTANSARLEVERD NLAQDLATVRQKLQD ETNLRLEAENNLAAY RQEADEATLARLDLE RKIESLEEEIRFLRK IHEEEVRELQEQLAR QQVHVELDVAKPDLT AALKEIRTQYEAMAS SNMHEAEEWYRSKFA DLTDAAARNAELLRQ AKHEANDYRRQLQSL TCDLESLRGTNESLE RQMREQEERHVREAA SYQEALARLEEEGQS LKDEMARHLQEYQDL LNVKLALDIEIATYR KLLEGEENRITIPVQ TFSNLQIRETSLDTK SVSEGHLKRNIVVKT VEMRDGEVIKESKQE HKDVM|
|Endotoxin :||<1.0 EU per μg protein as determined by the LAL method.|
|Purity :||85%, by SDS-PAGE with Coomassie Brilliant Blue staining.|
|Applications :||Blocking peptide|
|Stability :||Store for up to 12 months at -20°C to -80°C as lyophilized powder.|
|Storage :||Short-term storage: Store at 2-8°C for (1-2 weeks). Long-term storage: Aliquot and store at -20°C to -80°C for up to 3 months, buffer containing 50% glycerol is recommended for reconstitution. Avoid repeat freeze-thaw cycles.|
Reconstitute at 0.25 µg/μl in 200μl sterile water for short-term storage.
Reconstitution with 200μl 50% glycerol solution is recommended for longer term storage (see Stability and Storage for more details).
If a different concentration is needed for your purposes please adjust the reconstitution volume as required (please note: the ion concentration of the final solution will vary according to the volume used).
Note: Centrifuge vial before opening. When reconstituting, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution.
|Shipping :||The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature (see below).|
|SDS-PAGE of GFAP-1307H :||
|Gene Name :||GFAP|
|Official Symbol :||GFAP|
|Gene ID :||2670|
|mRNA Refseq :||NM_001131019.3|
|Protein Refseq :||NP_001124491.1|
|UniProt ID :||P14136|
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