Recombinant Human GJA1 Protein, His-tagged

• Cat.No.: GJA1-2204H
• Product Overview: Recombinant Human GJA1 Protein (Ser180-Ile382) with a N-His tag was expressed in E. coli.

Specification

• Description: This gene is a member of the connexin gene family. The encoded protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. The encoded protein is the major protein of gap junctions in the heart that are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. A related intronless pseudogene has been mapped to chromosome 5. Mutations in this gene have been associated with oculodentodigital dysplasia, autosomal recessive craniometaphyseal dysplasia and heart malformations.
• Source: E. coli
• Species: Human
• Tag: N-His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 42.7 kDa
  Accurate Molecular Mass: 40 kDa
• Protein length: Ser180-Ile382
• Endotoxin: <1.0 EU per 1µg (determined by the LAL method).
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: GJA1 gap junction protein alpha 1 [ Homo sapiens (human) ]
• Official Symbol: GJA1
• Synonyms: GJA1; gap junction protein alpha 1; HSS; CMDR; CX43; EKVP; GJAL; ODDD; AVSD3; EKVP3; HLHS1; PPKCA; gap junction alpha-1 protein
  connexin-43
  gap junction 43 kDa heart protein
  gap junction protein, alpha 1, 43kDa
• Gene ID: 2697
• mRNA Refseq: NM_000165
• Protein Refseq: NP_000156
• MIM: 121014
• UniProt ID: P17302