Recombinant Human GLP1R Protein, hFc-tagged

• Cat.No.: GLP1R-583H
• Product Overview: Recombinant Human GLP1R Protein(NP_002053.3) (Met1-Tyr145) was expressed in HEK293 with the Fc region of human IgG1 at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: 1-145 aa
• Form: Lyophilized from sterile PBS, pH 7.4.
• Molecular Mass: The recombinant human GLP1R consists 363 amino acids and predicts a molecular mass of 41.3 kDa.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE.
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: GLP1R glucagon-like peptide 1 receptor [ Homo sapiens ]
• Official Symbol: GLP1R
• Synonyms: GLP1R; glucagon-like peptide 1 receptor; GLP-1R; GLP-1-R; GLP-1 receptor; MGC138331
• Gene ID: 2740
• mRNA Refseq: NM_002062
• Protein Refseq: NP_002053
• MIM: 138032
• UniProt ID: P43220

Citation

Loureirin B activates GLP‐1R and promotes insulin secretion in Ins‐1 cells

Journal: Journal of Cellular and Molecular Medicine    PubMed ID: 33300675    Data: 2020/12/10

Authors: Yanting Ding, Sijing Xia, Bing Niu

Article Snippet:The recombinant protein GLP‐1R was obtained from Creative BioMart.. Loureirin B (LB) was purchased from National Institutes for Food and Drug Control.Loureirin B (LB) was purchased from National Institutes for Food and Drug Control.

Molecular docking results of LB and DPP‐IV, FFAR1, GK, GLP‐1R

Molecular docking diagram of LB interaction with GLP‐1R.(A) 3D structure of interaction between LB and GLP‐1R.(B) Hydrogen bonding between LB and GLP‐1R.(C) 2D structure of interaction between LB and GLP‐1R.(D) Hydrophobic interaction between LB and GLP‐1R

SPR sensorgrams corresponding to different concentrations LB and GLP‐1R. (A) LB was diluted with running buffer to different concentrations and then sampled and captured on the chip channel surface (4 × 10 ‐5 mol/L‐7.8 × 10 ‐8 mol/L). (B) The detection channel LB (a‐e:2 × 10 ‐5 mol/L‐1.25 × 10 ‐6 mol/L) was sampled and captured on the chip channel surface

Cochinchinenin C, a potential nonpolypeptide anti-diabetic drug, targets a glucagon-like peptide-1 receptor

Journal: RSC Adv.    Data: 2017/1/1

Authors: Yijie Sha, Yunfeng Shi, Qin Chen

Article Snippet:The recombinant protein GLP1 receptor was purchased from Creative BioMart and used without further purication, congured as 25 mM.. Cochinchinenin C was purchased from National Institutes for Food and Drug Control, and the stock 49016 | RSC Adv., 2017, 7, 49015–49023 solution of Cochinchinenin C (CC) was prepared in DMSO at 0.01 mol L 1.Cochinchinenin C was purchased from National Institutes for Food and Drug Control, and the stock 49016 | RSC Adv., 2017, 7, 49015–49023 solution of Cochinchinenin C (CC) was prepared in DMSO at 0.01 mol L 1.