Recombinant Human GLP1R Protein, hFc-tagged

Cat.No. : GLP1R-583H
Product Overview : Recombinant Human GLP1R Protein(P43220)(Arg24-Tyr145), fused with C-terminal hFc tag, was expressed in HEK293.
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Species : Human
Source : HEK293
Tag : Fc
Protein Length : 1-145 a.a.
Tag : C-hFc
Form : Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Bio-activity : Immobilized Recombinant Human GLP-1R (C-Fc) at 5μg/ml (100 μl/well) can bind Anti-GLP-1R Antibody (Human IgG1). The ED50 of Anti-GLP-1R Antibody (Human IgG1) is 6.23 ng/ml.
Molecular Mass : 41.2 KDa
Endotoxin : < 1 EU/μg as determined by LAL test.
Purity : Greater than 90% as determined by reducing SDS-PAGE.
Storage : Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.
Reconstituted protein solution can be stored at 2-8°C for 2-7 days.
Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Reconstitution : Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Gene Name GLP1R glucagon-like peptide 1 receptor [ Homo sapiens ]
Official Symbol GLP1R
Synonyms GLP1R; glucagon-like peptide 1 receptor; GLP-1R; GLP-1-R; GLP-1 receptor; MGC138331;
Gene ID 2740
mRNA Refseq NM_002062
Protein Refseq NP_002053
MIM 138032
UniProt ID P43220

Loureirin B activates GLP‐1R and promotes insulin secretion in Ins‐1 cells

Journal: Journal of Cellular and Molecular Medicine    PubMed ID: 33300675    Data: 2020/12/10

Authors: Yanting Ding, Sijing Xia, Bing Niu

Article Snippet:The recombinant protein GLP‐1R was obtained from Creative BioMart.. Loureirin B (LB) was purchased from National Institutes for Food and Drug Control.Loureirin B (LB) was purchased from National Institutes for Food and Drug Control.

Molecular docking results of LB and DPP‐IV, FFAR1, GK,  GLP‐1R

Molecular docking results of LB and DPP‐IV, FFAR1, GK, GLP‐1R

Molecular docking diagram of LB interaction with GLP‐1R.(A) 3D structure of interaction between LB and GLP‐1R.(B) Hydrogen bonding between LB and GLP‐1R.(C) 2D structure of interaction between LB and GLP‐1R.(D) Hydrophobic interaction between LB and GLP‐1R

Molecular docking diagram of LB interaction with GLP‐1R.(A) 3D structure of interaction between LB and GLP‐1R.(B) Hydrogen bonding between LB and GLP‐1R.(C) 2D structure of interaction between LB and GLP‐1R.(D) Hydrophobic interaction between LB and GLP‐1R

SPR sensorgrams corresponding to different concentrations LB and GLP‐1R. (A) LB was diluted with running buffer to different concentrations and then sampled and captured on the chip channel surface (4 × 10 ‐5 mol/L‐7.8 × 10 ‐8 mol/L). (B) The detection channel LB (a‐e:2 × 10 ‐5 mol/L‐1.25 × 10 ‐6 mol/L) was sampled and captured on the chip channel surface

SPR sensorgrams corresponding to different concentrations LB and GLP‐1R. (A) LB was diluted with running buffer to different concentrations and then sampled and captured on the chip channel surface (4 × 10 ‐5 mol/L‐7.8 × 10 ‐8 mol/L). (B) The detection channel LB (a‐e:2 × 10 ‐5 mol/L‐1.25 × 10 ‐6 mol/L) was sampled and captured on the chip channel surface

Cochinchinenin C, a potential nonpolypeptide anti-diabetic drug, targets a glucagon-like peptide-1 receptor

Journal: RSC Adv.    Data: 2017/1/1

Authors: Yijie Sha, Yunfeng Shi, Qin Chen

Article Snippet:The recombinant protein GLP1 receptor was purchased from Creative BioMart and used without further purication, congured as 25 mM.. Cochinchinenin C was purchased from National Institutes for Food and Drug Control, and the stock 49016 | RSC Adv., 2017, 7, 49015–49023 solution of Cochinchinenin C (CC) was prepared in DMSO at 0.01 mol L 1.Cochinchinenin C was purchased from National Institutes for Food and Drug Control, and the stock 49016 | RSC Adv., 2017, 7, 49015–49023 solution of Cochinchinenin C (CC) was prepared in DMSO at 0.01 mol L 1.

Not For Human Consumption!

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