Recombinant Human GLRX Protein, His-tagged

• Cat.No.: GLRX-139H
• Product Overview: Active human recombinant N-terminal His-TEV tagged Grx1 (2-106) was expressed in E. coli.

Specification

• Description: This gene encodes a member of the glutaredoxin family. The encoded protein is a cytoplasmic enzyme catalyzing the reversible reduction of glutathione-protein mixed disulfides. This enzyme highly contributes to the antioxidant defense system. It is crucial for several signalling pathways by controlling the S-glutathionylation status of signalling mediators. It is involved in beta-amyloid toxicity and Alzheimer's disease. Multiple alternatively spliced transcript variants encoding the same protein have been identified.
• Source: E. coli
• Species: Human
• Tag: His
• Molecular Mass: 13.9 kDa
• Purity: ≥ 95% estimated by SDS-PAGE
• Unit Definition: One unit is defined as the amount of enzyme required to produce 1 nmol of Eosin-GSH per minute at 25 centigrade in 0,1 Mpotassium phosphate, pH 7.5, containing 1 mM EDTA, 1U/mL glutathione reductase, 0.5 mM GSH, 0.25 mM NADPH, 0.75 μM alkylated BSA, and 20 μM Eosin-GS-BSA.
• Applications: Enzyme Activity
• Stability: ≥ 1 year
• Storage: At -80 centigrade.
• Storage Buffer: 50 mM Tris-HCl, pH 7.8, with 150 mM sodium chloride, 10% glycerol, 1 mM EDTA, and 0.1 mM DTT

Gene Information

• Gene Name: GLRX glutaredoxin [ Homo sapiens (human) ]
• Official Symbol: GLRX
• Synonyms: GLRX; glutaredoxin; GRX; GRX1; glutaredoxin-1; TTase-1; thioltransferase; thioltransferase-1; EC 1.8.4.1; tyrosine-protein kinase Blk; B lymphoid tyrosine kinase; BLK nonreceptor tyrosine kinase; b lymphocyte kinase; p55-Blk; EC 2.7.10.2
• Gene ID: 2745
• mRNA Refseq: NM_002064
• Protein Refseq: NP_002055
• MIM: 600443
• UniProt ID: P35754