Recombinant Human GLS protein, His-tagged

• Cat.No.: GLS-1608H
• Product Overview: Recombinant Human GLS protein (370-669aa) was fused to His-tag and expressed in E.coli.

Specification

• Description: This gene encodes the K-type mitochondrial glutaminase. The encoded protein is an phosphate-activated amidohydrolase that catalyzes the hydrolysis of glutamine to glutamate and ammonia. This protein is primarily expressed in the brain and kidney plays an essential role in generating energy for metabolism, synthesizing the brain neurotransmitter glutamate and maintaining acid-base balance in the kidney. Alternate splicing results in multiple transcript variants.
• Source: E.coli
• Species: Human
• Tag: His
• Form: The purified protein was Lyophilized from sterile PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH7.4). 5% trehalose and 5% mannitol are added as protectants before lyophilization.
• Molecular Mass: 35 kDa
• AA Sequence: YVGFSNATFQSERESGDRNFAIGYY LKEKKCFPEGTDMVGILDFYFQLCS IEVTCESASVMAATLANGGFCPITG ERVLSPEAVRNTLSLMHSCGMYDFS GQFAFHVGLPAKSGVAGGILLVVPN VMGMMCWSPPLDKMGNSVKGIHFCH DLVSLCNFHNYDNLRHFAKKLDPRR EGGDQRVKSVINLLFAAYTGDVSAL RRFALSAMDMEQRDYDSRTALHVAA AEGHVEVVKFLLEACKVNPFPKDRW NNTPM
• Purity: 90%, by SDS-PAGE with Coomassie Brilliant Blue staining.
• Storage: Store for up to 12 months at -20 to -80 centigrade as lyophilized powder. Short-term storage: Store at 2-8 centigrade for (1-2 weeks). Long-term storage: Aliquot and store at -20 to -80 centigrade for up to 3 months, buffer containing 50% glycerol is recommended for reconstitution. Avoid repeat freeze-thaw cycles.
• Reconstitution: Reconstitute at 0.25 µg/μl in 200 μl sterile water for short-term storage.
  Reconstitution with 200 μl 50% glycerol solution is recommended for longer term storage.
  If a different concentration is needed for your purposes please adjust the reconstitution
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Gene Information

• Gene Name: GLS
• Official Symbol: GLS
• Synonyms: GAC; GAM; KGA; GLS1; AAD20; GDPAG; CASGID; EIEE71
• Gene ID: 2744
• mRNA Refseq: NM_014905.5
• Protein Refseq: NP_055720.3
• MIM: 138280
• UniProt ID: O94925