Recombinant Human glutamyl-tRNA(Gln) amidotransferase, subunit C, His-tagged

• Cat.No.: GATC-229H
• Product Overview: GATC, 1-136aa, Human, His tag, E.coli

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-136 a.a.
• Description: GATC allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in the mitochondria. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln). This protein is subunit of the heterotrimeric GatCAB amidotransferase (AdT) complex, composed of A (QRSL1), B (PET112) and C (GATC) subunits. Recombinant human GATC protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 30% glycerol, 1mM DTT
• Molecular Mass: 17.5 kDa (159aa), confirmed by MALDI-TOF(Molecular size on SDS-PAGE will appear higher)
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMWSRLVW LGLRAPLGGR QGFTSKADPQ GSGRITAAVI EHLERLALVD FGSREAVARL EKAIAFADRL RAVDTDGVEP MESVLEDRCL YLRSDNVVEG NCADELLQNS HRVVEEYFVA PPGNISLPKL DEQEPFPHS
• Purity: >85% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: GATC?glutamyl-tRNA(Gln) amidotransferase, subunit C [?Homo sapiens(human) ]
• Official Symbol: GATC
• Synonyms: GATC; 15E1.2; glutamyl-tRNA(Gln) amidotransferase, subunit C; glutamyl-tRNA(Gln) amidotransferase subunit C, mitochondrial; glu-AdT subunit C; glutamyl-tRNA(Gln) amidotransferase, subunit C homolog; NP_789788.1; EC 6.3.5.-
• Gene ID: 283459
• mRNA Refseq: NM_176818
• Protein Refseq: NP_789788
• UniProt ID: O43716
• Chromosome Location: 12q24.31
• Pathway: Aminoacyl-tRNA biosynthesis; mRNA processing
• Function: ATP binding; glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity