Recombinant Human GMCSF, His-tagged

• Cat.No.: CSF2-85H
• Product Overview: Recombinant Human GMCSF produced inE.Coliis a single, non-glycosylated, polypeptide chain containing 127 amino acids fragment (18-144) and having a molecular mass of 18.98 KDa Dalton with an amino-terminal hexahistidine tag. Recombinant Human GM-CSF His is purified by proprietary chromatographic techniques.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 18-144 a.a.
• Description: GMCSF is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13. GM-CSF stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.
• Physical Appearance: Sterile Filtered clear solution.
• Purity: Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Formulation: Granulocyte Macrophage Colony Stimulating Factor-His supplied in 20mM Tris HCl (pH 8) and 50% glycerol.
• Solubility: It is recommended to reconstitute the lyophilized Granulocyte Macrophage Colony Stimulating Factor in sterile 18 MΩ-cm H₂O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
• Stability: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Please avoid freeze thaw cycles.

Gene Information

• Synonyms: CSF; GM-CSF; GMCSF; MGC131935; MGC138897; CSF2; CSF-2; MGI-1GM; Pluripoietin-alpha; Molgramostin; Sargramostim; molgramostin; sargramostim; colony stimulating factor 2; Colony-stimulating factor; granulocyte-macrophage colony stimulating factor; colony stimulating factor 2 (granulocyte-macrophage)
• Gene Name: CSF2 colony stimulating factor 2 (granulocyte-macrophage) [ Homo sapiens ]
• Gene ID: 1437
• mRNA Refseq: NM_000758
• Protein Refseq: NP_000749
• MIM: 138960
• UniProt ID: P04141
• Chromosome Location: 5q31.1
• Pathway: Cytokine-cytokine receptor interaction; Fc epsilon RI signaling pathway; Hematopoietic cell lineage; Jak-STAT signaling pathway; Natural killer cell mediated cytotoxicity; T cell receptor signaling pathway
• Function: cytokine activity; granulocyte macrophage colony-stimulating factor receptor binding; growth factor activity; protein binding