Recombinant Human GPI Protein, His-tagged

• Cat.No.: GPI-059H
• Product Overview: Recombinant C-terminal His-tagged human GPI protein (2-558) was expressed in E. coli.

Specification

• Description: This gene encodes a member of the glucose phosphate isomerase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. In the cytoplasm, the gene product functions as a glycolytic enzyme (glucose-6-phosphate isomerase) that interconverts glucose-6-phosphate and fructose-6-phosphate. Extracellularly, the encoded protein (also referred to as neuroleukin) functions as a neurotrophic factor that promotes survival of skeletal motor neurons and sensory neurons, and as a lymphokine that induces immunoglobulin secretion. The encoded protein is also referred to as autocrine motility factor based on an additional function as a tumor-secreted cytokine and angiogenic factor. Defects in this gene are the cause of nonspherocytic hemolytic anemia and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment. Alternative splicing results in multiple transcript variants.
• Source: E. coli
• Species: Human
• Tag: His
• Molecular Mass: 66.0 kDa
• Protein length: 2-558
• Purity: ≥95% as determined by SDS-PAGE
• Unit Definition: One unit is defined as the amount of enzyme required to reduce 1 μmol of NADP+ to NADPH per minute at 25 centigrade in 50 mM Triethanolamine, pH7.6, containing 1 mM MgCl2.
• Stability: ≥ 2 years
• Storage: At -80 centigrade.
• Storage Buffer: 25 mM Tris-HCl, pH 7.0, 100 mM sodium chloride, and 10% glycerol

Gene Information

• Gene Name: GPI glucose-6-phosphate isomerase [ Homo sapiens (human) ]
• Official Symbol: GPI
• Synonyms: GPI; glucose-6-phosphate isomerase; AMF; NLK; PGI; PHI; GNPI; SA36; SA-36; glucose-6-phosphate isomerase; autocrine motility factor; hexose monophosphate isomerase; hexosephosphate isomerase; neuroleukin; oxoisomerase; phosphoglucose isomerase; phosphohexomutase; phosphohexose isomerase; phosphosaccharomutase; sperm antigen-36; EC 5.3.1.9
• Gene ID: 2821
• mRNA Refseq: NM_000175
• Protein Refseq: NP_000166
• MIM: 172400
• UniProt ID: P06744