Recombinant Human GPI Protein, GST-tagged

• Cat.No.: GPI-3928H
• Product Overview: Recombinant Human GPI Protein (Ala2-Gln558) is produced by E. Coli expression system. This protein is fused with a GST tag at the N-terminus.

Specification

• Description: Glucose-6-phosphate isomerase (GPI), is also known as Autocrine motility factor (AMF), Neuroleukin (NLK), Phosphoglucose isomerase (PGI), Phosphohexose isomerase (PHI), Sperm antigen 36 (SA-36). GPI belongs to the GPI family. GPI is homodimer in the catalytically active form and is monomer in the secreted form. GPI can catalyze D-glucose 6-phosphate to be D-fructose 6-phosphate. Besides it's role as a glycolytic enzyme, mammalian GPI can function as a tumor-secreted cytokine and an angiogenic factor (AMF) that stimulates endothelial cell motility. GPI is also a neurotrophic factor (Neuroleukin) for spinal and sensory neurons.
• Source: E. coli
• Species: Human
• Tag: GST
• Form: Protein in [50 mM Tris, pH7.5 with 10 mM GSH, 1 mM EDTA] was 0.22 um filtered before lyophilized. Usually protectant (mannitol or trehalose) was added before lyophilization.
• Molecular Mass: 86.8 kDa
• Protein length: Ala2-Gln558
• Endotoxin: < 1.0 EU/ug by LAL method
• Purity: >95% as determined by SDS-PAGE
• Notes: All products are For Research Use Only, and not for diagnostic or therapeutic purposes.
• Storage: Lyophilized form is stable at room temperature for 3 weeks. Keep at -20 centigrade for long term storage. After reconstitution, store at 4 centigrade for 2-7 days or at below -20 centigrade for up to 3 months.
• Reconstitution: Reconstitute lyophilized powder with water to no less than 100 ug/ml, solubilize at RT for 30 min with occasional gentle mixing. It is recommended that 0.1% HSA (SDP1139) be added as a carrier protein for long term storage.
• Shipping: Ship at ambient conditions

Gene Information

• Gene Name: GPI glucose-6-phosphate isomerase [ Homo sapiens ]
• Official Symbol: GPI
• Synonyms: GPI; AMF; NLK; neuroleukin; oxoisomerase; sperm antigen 36; sperm antigen-36; phosphohexomutase; phosphosaccharomutase; PGI; PHI; GNPI; SA36; SA-36; DKFZp686C13233
• Gene ID: 2821
• mRNA Refseq: NM_000175
• Protein Refseq: NP_000166
• MIM: 172400
• UniProt ID: P06744

Reviews

11/25/2020

Its impressive performance and diverse applications make it an invaluable tool for a wide range of research endeavors.

02/25/2019

The GPI Protein is a top-quality protein that perfectly meets the demands of my experimental requirements.

09/25/2017

The bands of proteins in Western Blotting experiments were very clear.

Q&As

How does the study of GPI protein contribute to understanding rare genetic disorders?

Mutations in genes related to GPI protein synthesis can lead to rare genetic disorders, and studying GPI proteins helps in understanding the underlying mechanisms of these conditions.

Are there any side effects or risks associated with manipulating GPI protein levels for therapeutic purposes?

Modulating GPI protein levels may have implications for cell function, and potential side effects and risks are part of ongoing research in therapeutic development.

How does the study of GPI protein contribute to understanding autoimmune diseases?

Abnormalities in GPI-anchored proteins have been linked to autoimmune diseases, providing valuable information for understanding the underlying causes and potential treatment strategies.

In what ways does GPI protein impact cancer research and treatment?

GPI proteins are implicated in cancer cell growth and metastasis, making them a target for cancer research and potential therapeutic strategies.

How does GPI protein contribute to neurological disorders, and what are its implications in clinical research?

Abnormalities in GPI-anchored proteins have been linked to neurological disorders, making them a focus of clinical research for potential therapeutic interventions.