Recombinant Human GRK4 Protein
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|Product Overview :||Human GRK4 (NP_892027.2, 1 a.a. - 578 a.a.) full-length recombinant protein expressed in baculovirus infected Sf21 cells.|
|Description :||This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates the activated forms of G protein-coupled receptors thus initiating its deactivation. This gene has been linked to both genetic and acquired hypertension. [provided by RefSeq|
|Source :||Insect Cell|
|Bio-activity :||The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip 3000.
Substrate: Modified PLKtide.
ATP: 100 μM.
|Molecular Mass :||67 kDa|
|Purity :||78 % by SDS-PAGE/CBB staining|
|Applications :||Functional Study
|Storage :||Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.|
|Storage Buffer :||In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.1% CHAPS, 1 mM DTT, 10% glycerol)|
|Quality Control Testing :||
Loading 1 μg protein in SDS-PAGE
|Result of activity analysis :||
|Gene Name :||GRK4 G protein-coupled receptor kinase 4 [ Homo sapiens ]|
|Official Symbol :||GRK4|
|Synonyms :||GRK4; G protein-coupled receptor kinase 4; G protein coupled receptor kinase 2 like (Drosophila), GPRK2L; GPRK4; ITI1; IT11; GRK4a; GPRK2L;|
|Gene ID :||2868|
|mRNA Refseq :||NM_001004056|
|Protein Refseq :||NP_001004056|
|UniProt ID :||P32298|
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