Active Recombinant Human GRK4 Protein

• Cat.No.: GRK4-5353H
• Product Overview: Human GRK4 (NP_892027.2, 1 a.a. - 578 a.a.) full-length recombinant protein expressed in baculovirus infected Sf21 cells.

Specification

• Description: This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates the activated forms of G protein-coupled receptors thus initiating its deactivation. This gene has been linked to both genetic and acquired hypertension. [provided by RefSeq
• Source: Insect Cell
• Species: Human
• Form: Liquid
• Bio-activity: The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip 3000.
  Substrate: Modified PLKtide.
  ATP: 100 μM.
• Molecular Mass: 67 kDa
• Purity: 78 % by SDS-PAGE/CBB staining
• Applications: Functional Study
  SDS-PAGE
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.1% CHAPS, 1 mM DTT, 10% glycerol)

Gene Information

• Gene Name: GRK4 G protein-coupled receptor kinase 4 [ Homo sapiens ]
• Official Symbol: GRK4
• Synonyms: GRK4; G protein-coupled receptor kinase 4; G protein coupled receptor kinase 2 like (Drosophila), GPRK2L; GPRK4; ITI1; IT11; GRK4a; GPRK2L
• Gene ID: 2868
• mRNA Refseq: NM_001004056
• Protein Refseq: NP_001004056
• MIM: 137026
• UniProt ID: P32298