Recombinant Human GRK4 Protein

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Recombinant Human GRK4 Protein

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Cat.No. : GRK4-5353H
Product Overview : Human GRK4 (NP_892027.2, 1 a.a. - 578 a.a.) full-length recombinant protein expressed in baculovirus infected Sf21 cells.
Description : This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates the activated forms of G protein-coupled receptors thus initiating its deactivation. This gene has been linked to both genetic and acquired hypertension. [provided by RefSeq
Source : Insect Cell
Species : Human
Form : Liquid
Bio-activity : The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip 3000.
Substrate: Modified PLKtide.
ATP: 100 μM.
Molecular Mass : 67 kDa
Purity : 78 % by SDS-PAGE/CBB staining
Applications : Functional Study
SDS-PAGE
Storage : Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
Storage Buffer : In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.1% CHAPS, 1 mM DTT, 10% glycerol)
Quality Control Testing :

Loading 1 μg protein in SDS-PAGE

Result of activity analysis :

Gene Name : GRK4 G protein-coupled receptor kinase 4 [ Homo sapiens ]
Official Symbol : GRK4
Synonyms : GRK4; G protein-coupled receptor kinase 4; G protein coupled receptor kinase 2 like (Drosophila), GPRK2L; GPRK4; ITI1; IT11; GRK4a; GPRK2L;
Gene ID : 2868
mRNA Refseq : NM_001004056
Protein Refseq : NP_001004056
MIM : 137026
UniProt ID : P32298

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