| Cat.No. : |
GRK4-5353H |
| Product Overview : |
Human GRK4 (NP_892027.2, 1 a.a. - 578 a.a.) full-length recombinant protein expressed in baculovirus infected Sf21 cells. |
| Description : |
This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates the activated forms of G protein-coupled receptors thus initiating its deactivation. This gene has been linked to both genetic and acquired hypertension. [provided by RefSeq |
| Source : |
Insect Cell |
| Species : |
Human |
| Form : |
Liquid |
| Bio-activity : |
The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip 3000.
Substrate: Modified PLKtide.
ATP: 100 μM. |
| Molecular Mass : |
67 kDa |
| Purity : |
78 % by SDS-PAGE/CBB staining |
| Applications : |
Functional Study
SDS-PAGE |
| Storage : |
Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing. |
| Storage Buffer : |
In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.1% CHAPS, 1 mM DTT, 10% glycerol) |
| Quality Control Testing : |
Loading 1 μg protein in SDS-PAGE
|
| Result of activity analysis : |
|
