Recombinant Human GrpE-like 1, Mitochondrial (E. coli), His-tagged

• Cat.No.: GRPEL1-2840H
• Product Overview: Recombinant human GRPEL1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. MW =23.6 kDa (211aa) confirmed by MALDI-TOF.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: GRPEL1 is an essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner. This protein seems to control the nucleotide-dependent binding of mitochondrial HSP70 to substrate proteins.
• Sequences Of Amino Acids: MGSSHHHHHH SSGLVPRGSH MCTATKQKNS GQNLEEDMGQ SEQKADPPAT EKTLLEEKVK LEEQLKETVE KYKRALADTE NLRQRSQKLV EEAKLYGIQA FCKDLLEVAD VLEKATQCVP KEEIKDDNPH LKNLYEGLVM TEVQIQKVFT KHGLLKLNPV GAKFDPYEHE ALFHTPVEGK EPGTVALVSK VGYKLHGRTL RPALVGVVKE A
• Purity: > 90% by SDS – PAGE.
• Form: Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 0.2M NaCl, 1mM DTT, 10% glycerol.
• Concentration: 1 mg/ml (determined by Bradford assay).
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: GRPEL1 GrpE-like 1, mitochondrial (E. coli) [ Homo sapiens ]
• Synonyms: GRPEL1; GrpE-like 1, mitochondrial (E. coli); HMGE; FLJ25609; grpE protein homolog 1, mitochondrial; mt-GrpE#1; GrpE-like protein cochaperone
• Gene ID: 80273
• mRNA Refseq: NM_025196
• Protein Refseq: NP_079472
• MIM: 606173
• UniProt ID: Q9HAV7
• Chromosome Location: 4p16
• Function: adenyl-nucleotide exchange factor activity; chaperone binding; protein homodimerization activity; unfolded protein binding