Recombinant Human GTF2A1 protein, His & GST-tagged

• Cat.No.: GTF2A1-7865H
• Product Overview: Recombinant Human GTF2A1 aa. (Ala2~Asp274 (Accession # P52655)) fused with N-terminal His & GST tag was produced in E. coli cells.

Specification

• Description: Accurate transcription initiation on TATA-containing class II genes involves the ordered assembly of RNA polymerase II (POLR2A; MIM 180660) and several general initiation factors (summarized by DeJong and Roeder, 1993 [PubMed 8224848]). One of these factors is TFIIA, which when purified from HeLa extracts consists of 35-, 19-, and 12-kD subunits.
• Source: E. coli
• Species: Human
• Tag: His & GST
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 59.6kDa.
• Protein length: Ala2~Asp274 (Accession # P52655)
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 5.9.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage Buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: GTF2A1 general transcription factor IIA subunit 1 [ Homo sapiens (human) ]
• Official Symbol: GTF2A1
• Synonyms: GTF2A1; general transcription factor IIA subunit 1; TF2A1; TFIIA; TFIIAL; TFIIA-42; transcription initiation factor IIA subunit 1; TFIIA alpha, p55, isoform 1; TFIIA alpha/beta subunits; general transcription factor IIA 1; general transcription factor IIA, 1, 19/37kDa; glucose regulated protein, 58kD pseudogene; transcription initiation factor TFIIA 42 kDa subunit
• Gene ID: 2957
• mRNA Refseq: NM_001278940.1
• Protein Refseq: NP_001265869.1
• UniProt ID: P52655