Recombinant Human GTPase Activating Protein (SH3 Domain) Binding Protein 1

• Cat.No.: G3BP1-1295H
• Product Overview: G3BP1 Human Recombinant fused with 8 amino acid His tag at C-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 474 amino acids (1-466 a.a.) and having a molecular mass of 53.2kDa. The G3BP1 is purified by proprietary chromatographic techniques.

Specification

• Cat. No.: G3BP1-1295H
• Description: G3BP1 belongs to the heterogeneous nuclear RNA-binding proteins and is also an element of the Ras signal transduction pathway. G3BP1 is one of the DNA-unwinding enzymes that favors partially unwound 3"-tailed substrates and is also able to unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion. G3BP1 binds specifically to the Ras-GTPase-activating protein by associating with its SH3 domain. In addition, G3BP1 cleaves exclusively between cytosine and adenine and cleaves MYC mRNA preferentially at the 3"-UTR.
• Source: Human
• Host: Escherichia Coli.
• Form: The G3BP1 solution (0.5 mg/ml) contains 20mM Tris-HCl buffer (pH8.0), 10% glycerol, 2mM DTT and 100mM NaCl.
• Purity: Greater than 85.0% as determined by SDS-PAGE.
• Physical Appearance: Sterile Filtered colorless solution.
• Amino acid sequence: MVMEKPSPLL VGREFVRQYY TLLNQAPDML HRFYGKNSSY VHGGLDSNGK PADAVYGQKE IHRKVMSQNF TNCHTKIRHV DAHATLNDGV VVQVMGLLSN NNQALRRFMQ TFVLAPEGSV ANKFYVHNDI FRYQDEVFGG FVTEPQEESE EEVEEPEERQ QTPEVVPDDS GTFYDQAVVS NDMEEHLEEP VAEPEPDPEP EPEQEPVSEI QEEKPEPVLE ETAPEDAQKS SSPAPADIAQ TVQEDLRTFS WASVTSKNLP PSGAVPVTGI PPHVVKVPAS QPRPESKPES QIPPQRPQRD QRVREQRINI PPQRGPRPIR EAGEQGDIEP RRMVRHPDSH QLFIGNLPHE VDKSELKDFF QSYGNVVELR INSGGKLPNF GFVVFDDSEP VQKVLSNRPI MFRGEVRLNV EEKKTRAARE GDRRDNRLRG PGGPRGGLGG GMRGPPRGGM VQKPGFGVGR GLAPRQVEHH HHHH.
• Storage: G3BP1 should be stored desiccated below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Gene Information

• Gene Name: G3BP1 GTPase activating protein (SH3 domain) binding protein 1 [ Homo sapiens ]
• Official Symbol: G3BP1
• Synonyms: GTPase activating protein (SH3 domain) binding protein 1; G3BP; HDH-VIII; MGC111040; G3BP1; ras GTPase-activating protein-binding protein 1; G3BP-1; GAP binding protein; ATP-dependent DNA helicase VIII; GAP SH3 domain-binding protein 1; RasGAP-associated endoribonuclease G3BP; Ras-GTPase-activating protein SH3-domain-binding protein; hDH VIII; EC 3.6.1.
• Gene ID: 10146
• mRNA Refseq: NM_005754
• Protein Refseq: NP_005745
• MIM: 608431
• UniProt ID: Q13283
• Chromosome Location: 5q33.1
• Function: ATP binding; ATP-dependent DNA helicase activity; ATP-dependent RNA helicase activity; DNA binding; RNA binding; endonuclease activity; helicase activity; hydrolase activity; nucleotide binding protein binding