Recombinant Human Guanine Monphosphate Synthetase, His-tagged

• Cat.No.: GMPS-1927H
• Product Overview: Recombinant humanGMPS protein, fused to His-tag at N-terminus, was expressed in E. coli andpurified by using conventional chromatography techniques.

Specification

• Species: Human
• Tag: His
• Description: GMPsynthase, also known as GMPS, is involved in purine biosynthesis. Existing asa homodimer, GMPS catalyzes the last step in the GMP synthesis pathway,namely the ATP-dependent amination of XMP to GMP.
• Form: Liquid. In 20mMTris-HCl buffer (pH 8.0) containing 1mM DTT, 30% glycerol, 0.1M NaCl.
• Molecular Weight: 79.2 kDa(717aa)
• Purity: > 90%by SDS-PAGE
• Concentration: 0.5mg/ml (determined by Bradford assay)
• Sequences of aminoacids: MGSSHHHHHHSSGLVPRGSH MGSHMALCNG DSKLENAGGD LKDGHHHYEG AVVILDAGAQ YGKVIDRRVRELFVQSEIFP LETPAFAIKE QGFRAIIISG GPNSVYAEDA PWFDPAIFTIGKPVLGICYG MQMMNKVFGG TVHKKSVRED GVFNISVDNT CSLFRGLQKE EVVLLTHGDS VDKVADGFKVVARSGNIVAG IANESKKLYG AQFHPEVGLT ENGKVILKNF LYDIAGCSGT FTVQNRELEC IREIKERVGTSKVLVLLSGGVDSTVCTALLNRALNQEQVI AVHIDNGFMR KRESQSVEEA LKKLGIQVKV INAAHSFYNG TTTLPISDED RTPRKRISKTLNMTTSPEEKRKIIGDTFVKIANEVIGEMN LKPEEVFLAQ GTLRPDLIES ASLVASGKAE LIKTHHNDTE LIRKLREEGK VIEPLKDFHKDEVRILGRELGLPEELVSRHPFPGPGLAIR VICAEEPYIC KDFPETNNIL KIVADFSASV KKPHTLLQRV KACTTEEDQE KLMQITSLHSLNAFLLPIKT VGVQGDCRSY SYVCGISSKD EPDWESLIFL ARLIPRMCHN VNRVVYIFGP PVKEPPTDVTPTFLTTGVLS TLRQADFEAH NILRESGYAG KISQMPVILTPLHFDRDPLQ KQPSCQRSVV IRTFITSDFM TGIPATPGNE IPVEVVLKMV TEIKKIPGIS RIMYDLTSKPPGTTEWE
• Storage: Can bestored at +4°C short term (1-2 weeks). For long term storage, aliquot andstore at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Pathways: Drug metabolism-other enzymes; Metabolicpathways; Metabolism; Metabolism of nucleotides; Purine metabolism; Purineribonucleoside monophosphate biosynthesis

Gene Information

• Gene Name: GMPS guanine monphosphatesynthetase [ Homo sapiens ]
• Official Symbol: GMPS
• Synonyms: GMPS; guanine monphosphate synthetase; GMPsynthase [glutamine-hydrolyzing]; GMP synthase; GMP synthetase; MLL/GMPSfusion protein; glutamine amidotransferase; guanosine 5"-monophosphatesynthase; EC 6.3.5.2
• Gene ID: 8833
• mRNA Refseq: NM_003875
• Protein Refseq: NP_003866
• MIM: 600358
• UniProt ID: P49915
• Chromosome Location: 3q24
• Function: ATP binding; GMP synthase(glutamine-hydrolyzing) activity; GMP synthase activity; ligase activity;nucleotide binding