Recombinant Human HDAC1 Protein (1-482 aa), His-SUMO-tagged

• Cat.No.: HDAC1-549H
• Product Overview: Recombinant Human HDAC1 Protein (1-482 aa) is produced by E. coli expression system. This protein is fused with a 6xHis-SUMO tag at the N-terminal. Research Area: Immunology. Protein Description: Full Length.

Specification

• Description: Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Deacetylates SP proteins, SP1 and SP3, and regulates their function. Component of the BRG1-RB1-HDAC1 complex, which negatively regulates the CREST-mediated transcription in resting neurons. Upon calcium stimulation, HDAC1 is released from the complex and CREBBP is recruited, which facilitates transcriptional activation. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Deacetylates 'Lys-310' in RELA and thereby inhibits the transcriptional activity of NF-kappa-B. Deacetylates NR1D2 and abrogates the effect of KAT5-mediated relieving of NR1D2 transcription repression activity. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development. Involved in CIART-mediated transcriptional repression of the circadian transcriptional activator: CLOCK-ARNTL/BMAL1 heterodimer. Required for the transcriptional repression of circadian target genes, such as PER1, mediated by the large PER complex or CRY1 through histone deacetylation.
• Source: E. coli
• Species: Human
• Tag: His-SUMO
• Form: Tris-based buffer, 50% glycerol
• Molecular Mass: 71.1 kDa
• Protein length: 1-482 aa
• AA Sequence: MAQTQGTRRKVCYYYDGDVGNYYYG QGHPMKPHRIRMTHNLLLNYGLYRK MEIYRPHKANAEEMTKYHSDDYIKF LRSIRPDNMSEYSKQMQRFNVGEDC PVFDGLFEFCQLSTGGSVASAVKLN KQQTDIAVNWAGGLHHAKKSEASGF CYVNDIVLAILELLKYHQRVLYIDI DIHHGDGVEEAFYTTDRVMTVSFHK YGEYFPGTGDLRDIGAGKGKYYAVN YPLRDGIDDESYEAIFKPVMSKVME MFQPSAVVLQCGSDSLSGDRLGCFN LTIKGHAKCVEFVKSFNLPMLMLGG GGYTIRNVARCWTYETAVALDTEIP NELPYNDYFEYFGPDFKLHISPSNM TNQNTNEYLEKIKQRLFENLRMLPH APGVQMQAIPEDAIPEESGDEDEDD PDKRISICSSDKRIACEEEFSDSEE EGEGGRKNSSNFKKAKRVKTEDEKE KDPEEKKEVTEEEKTKEEKPEAKGV KEEVKLA
• Purity: > 90% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
• Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
• Concentration: A hardcopy of COA with concentration instruction is sent along with the products.

Gene Information

• Gene Name: HDAC1 histone deacetylase 1 [ Homo sapiens ]
• Official Symbol: HDAC1
• Synonyms: HDAC1; RPD3L1; GON 10; HD1; RPD3; GON-10; DKFZp686H12203
• Gene ID: 3065
• mRNA Refseq: NM_004964
• Protein Refseq: NP_004955
• MIM: 601241
• UniProt ID: Q13547