Active Recombinant Human HDAC8 Protein
Cat.No. : | HDAC8-314H |
Product Overview : | Recombinant Human HDAC8 Protein is produced by HEK293 cells expression system. |
- Specification
- Gene Information
- Related Products
Description : | Human Histone Deacetylase 8 (HDAC8) is a member of the class I Histone Deacetylases. HDACs are important enzymes for the transcriptional regulation of gene expression in eukaryotic cells. HDACs catalyze the removal of acetyl groups from lysines near the N-termini of histones. Human HDACs have been implicated in a variety of human diseases such as cardiomyopathy, osteodystrophy, neurodegenerative disorders, aging and cancer. Expression of HDAC8 is restricted to cells showing smooth muscle differentiation in normal human tissue and is a novel marker of smooth muscle differentiation. Like other class I and II HDAC members, the activity of HDAC8 is sensitive to HDAC inhibitor Trichostatin A (TSA). |
Source : | HEK293 cells |
Species : | Human |
Form : | Liquid, In 30 mM HEPES, 140 mM NaCl, 10 mM KCl, 3% glycerol, 0.25 mM TCEP, pH 7.5. |
Bio-activity : | Deacetylation activity was determined using the Boc-K(TFA)-AMC substrate. Reactions were carried out in a 384-well plate in 20 µL of reaction buffer comprising of 50 mM HEPES, 140 mM NaCl, 10 mM KCl, 1 mg/ml bovine serum albumin (BSA), and 1 mM TCEP, pH 7.4, for 30 min at 37°C. The reaction was stopped by addition of 20 µl of trypsin solution (2 mg/ml trypsin) and the fluorescence signal of released AMC was quantified using a fluorometer. The enzyme kinetic values were calculated by nonlinear regression analysis |
Molecular Mass : | 41 kDa |
Protein length : | amino acids 1–377 |
Purity : | ≥95% by SDS-PAGE |
Notes : | For Research Use Only! Not For Use in Humans. |
Storage : | Store at -80 centigrade. |
Handling : | Thaw on ice, and aliquot into smaller working quantities to avoid multiple freeze/thaw cycles. |
Gene Name : | HDAC8 histone deacetylase 8 [ Homo sapiens ] |
Official Symbol : | HDAC8 |
Synonyms : | HDAC8; histone deacetylase 8; HDACL1, histone deacetylase like 1; RPD3; histone deacetylase-like 1; HD8; CDA07; HDACL1; |
Gene ID : | 55869 |
mRNA Refseq : | NM_001166418 |
Protein Refseq : | NP_001159890 |
MIM : | 300269 |
UniProt ID : | Q9BY41 |
Products Types
◆ Recombinant Protein | ||
HDAC8-4650H | Recombinant Human HDAC8 Protein, GST-tagged | +Inquiry |
HDAC8-125H | Recombinant Human HDAC8 Protein, His-tagged | +Inquiry |
HDAC8-2473R | Recombinant Rat HDAC8 Protein, His (Fc)-Avi-tagged | +Inquiry |
HDAC8-26H | Recombinant Human HDAC8 Protein, His-tagged | +Inquiry |
HDAC8-27529TH | Recombinant Human HDAC8, His-tagged | +Inquiry |
◆ Lysates | ||
HDAC8-691HCL | Recombinant Human HDAC8 cell lysate | +Inquiry |
HDAC8-537MCL | Recombinant Mouse HDAC8 cell lysate | +Inquiry |
◆ Assay kits | ||
Kit-2253 | HDAC8 Activity Fluorometric Assay Kit | +Inquiry |
Kit-1744 | HDAC8 Fluorogenic Assay Kit | +Inquiry |
Kit-1961 | HDAC8 Inhibitor Screening Assay Kit | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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