Recombinant Human HIF1A

• Cat.No.: HIF1A-136H
• Product Overview: Recombinant Humanhypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) produced inE.Coliis a single, non-glycosylated polypeptide chain. The protein contains 298(530-826) amino acids and has a molecular mass of 32.8 kDa. The protein migrates as a 40 kDa band on SDS-PAGE. The HIF1-A is purified by proprietary chromatographic techniques.

Specification

• Cat. No.: HIF1A-136H
• Description: Hypoxia-inducible factor-1 (HIF-1), identified as one of the transcription factors, has been found to play an essential role in oxygen homeostasis. HIF-1 is a heterodimer composed of HIF-1β subunit and one of three subunits(Hif-1α, Hif-2( or Hif-3)). The activation of Hif-1( is closely associated with a variety of tumors and oncogenic pathways. Hif-1( consists of DNA binding domain(DBD domain), Dimerization domain and C-terminla regulatiory domains, including two transactivation domains(TAD), an oxygen-dependent degradation(ODD) domain, and inhibitory domains.
• Source: Escherichia Coli.
• Amino Acid Sequence: MEFKLELVEK LFAEDTEAKN PFSTQDTDLD LEMLAPYIPM DDDFQLRSFD QLSPLESSSA SPESASPQST VTVFQQTQIQ EPTANATTTT ATTDELKTVT KDRMEDIKIL IASPSPTHIH KETTSATSSP YRDTQSRTAS PNRAGKGVIE QTEKSHPRSP NVLSVALSQR TTVPEEELNP KILALQNAQR KRKMEHDGSL FQAVGIGTLL QQPDDHAATT SLSWKRVKGC KSSEQNGMEQ KTIILIPSDL ACRLLGQSMD ESGLPQLTSY DCEVNAPIQG SRNLLQGEEL LRALDQVN.
• Physical Appearance: Sterile filtered liquid formulation 1mg/ml.
• Formulation: The HIF1A recombinant Human solution is formulated 20mM Tris pH-7.5 and 1mM DTT.
• Purity: Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Stability: Recombinant Human MOP1 although stable at 4°C for 30 days, should be stored desiccated below -20°C for periods greater than 30 days. Please avoid freeze-thaw cycles.

Gene Information

• Gene Name: HIF1A hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) [ Homo sapiens ]
• Synonyms: HIF1A; hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor); HIF1; MOP1; PASD8; bHLHe78; HIF-1alpha; HIF1-ALPHA; Hypoxia-inducible factor 1 alpha;HIF1 alpha; ARNT-interacting protein; Member of PAS protein 1; Basic-helix-loop-helix-PAS protein; ARNT interacting protein; hypoxia-inducible factor 1 alpha isoform I.3; member of PAS superfamily 1; hypoxia-inducible factor 1, alpha subunit
• Gene ID: 3091
• mRNA Refseq: NM_001530
• Protein Refseq: NP_001521
• MIM: 603348
• UniProt ID: Q16665
• Chromosome Location: 14q21-q24
• Pathway: Pathways in cancer; Renal cell carcinoma; mTOR signaling pathway
• Function: Hsp90 protein binding; RNA polymerase II transcription factor activity, enhancer binding; histone acetyltransferase binding; protein heterodimerization activity; sequence-specific DNA binding; signal transducer activity; transcription factor binding