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Recombinant Human HIST2H3A protein, His-tagged

Cat.No. : HIST2H3A-1559H
Product Overview : Recombinant Human HIST2H3A aa. (Met1~Ala136 (Accession # Q71DI3)) fused with N-terminal His tag was produced in E. coli cells.
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  • Gene Information
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Description : Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the centromeric copy.
Source : E. coli
Species : Human
Tag : His
Form : Freeze-dried powder
Molecular Mass : Predicted Molecular Mass: 16.9kDa
Protein length : Met1~Ala136 (Accession # Q71DI3)
Endotoxin : <1.0EU per 1ug (determined by the LAL method)
Purity : >95%
Applications : SDS-PAGE; WB; ELISA; IP.
Stability : The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage : Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
Storage buffer : Supplied as lyophilized form in PBS, pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Reconstitution : Reconstitute in sterile PBS, pH7.2-pH7.4.
Isoelectric Point : 11.1
Gene Name : HIST2H3A histone cluster 2 H3 family member a [ Homo sapiens (human) ]
Official Symbol : HIST2H3A
Synonyms : HIST2H3A; histone cluster 2 H3 family member a; H3/n; H3/o; histone H3.2; histone 2, H3a; histone H3/o; histone cluster 2, H3a
Gene ID : 333932
mRNA Refseq : NM_001005464.2
Protein Refseq : NP_001005464.1
UniProt ID : Q71DI3

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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