"HIST3H3" Related Products

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Recombinant Human HIST3H3 protein, His-tagged

Cat.No.: HIST3H3-290H
Product Overview: Recombinant Human HIST3H3(2-137 aa) fused with His tag at N-terminal was expressed in E. coli.
Description: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
Source: E. coli
Species: Human
Tag: His
Form: Formulated in 10 mM PBS, pH 7.4, 130 mM NaCl, 10% glycerol and 1 mM DTT.
Molecular Mass: 15.4 kDa
Purity: > 80% (SDS-PAGE)
Applications: Suitable substrate for histone methyltransferases
Storage: Thaw on ice. Upon first thaw, briefly spin tube containing protein to recover full content of the tube. Aliquot protein into single use aliquots. Store remaining undiluted protein in aliquots at -70 centigrade. Note: Protein is very sensitive to freeze/thaw cycles.
Gene Name: HIST3H3 histone cluster 3, H3 [ Homo sapiens ]
Official Symbol: HIST3H3
Synonyms: HIST3H3; histone cluster 3, H3; H3 histone family, member T , H3FT, histone 3, H3; histone H3.1t; H3/g; H3t; H3/t; histone 3, H3; H3 histone family, member T; H3.4; H3FT; MGC126886; MGC126888;
Gene ID: 8290
mRNA Refseq: NM_003493
Protein Refseq: NP_003484
MIM: 602820
UniProt ID: Q16695
Chromosome Location: 1q42.13
Pathway: Cell Cycle, organism-specific biosystem; Chromosome Maintenance, organism-specific biosystem; EGFR1 Signaling Pathway, organism-specific biosystem; Meiosis, organism-specific biosystem; Meiotic Recombination, organism-specific biosystem; Meiotic Synapsis, organism-specific biosystem; Packaging Of Telomere Ends, organism-specific biosystem;
Function: DNA binding; protein binding;

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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