Recombinant Human Histidyl-tRNA synthetase

• Cat.No.: HARS-46H
• Product Overview: Recombinant Human Histidyl-tRNA Synthetase produced inE.Coliis a single, non-glycosylated, polypeptide chain having a molecular mass of 55 kDa. The Histidyl-tRNA Synthetase is purified by proprietary chromatographic techniques.

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Description: Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is a cytoplasmic enzyme which belongs to the class II family of aminoacyl-tRNA synthetases. The enzyme is responsible for the synthesis of histidyl-transfer RNA, which is essential for the incorporation of histidine into proteins. The gene is located in a head-to-head orientation with HARSL on chromosome five, where the homologous genes share a bidirectional promoter. The gene product is a frequent target of autoantibodies in the human autoimmune disease polymyositis/dermatomyositis.
• Physical Appearance: Sterile Filtered clear solution.
• Purity: Greater than 90.0% as determined by both: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Formulation: The protein solution (0.74 mg/ml) contains 150 mM NaCl and 10 mM sodium phosphate containing 0.1% NaN3 (pH 7.2).
• Protein Content: Protein quantitation was carried out by using 0.25 - 2.0 mg/ml Bradford assay vs. BSA.
• Western Blot: Strongly reactive with human anti Histidyl-tRNA Synthetase antisera.
• Stability: Lyophilized Histidyl-tRNA Synthetase although stable at 4°C for 3 weeks, should be stored desiccated below -18°C. Please prevent freeze-thaw cycles.

Gene Information

• Gene Name: HARS histidyl-tRNA synthetase [ Homo sapiens ]
• Synonyms: HARS; histidyl-tRNA synthetase; HRS; FLJ20491; histidine tRNA ligase 1, cytoplasmic; EC 6.1.1.21; HisRS; histidine-tRNA ligase
• Gene ID: 3035
• mRNA Refseq: NM_002109
• Protein Refseq: NP_002100
• MIM: 142810
• UniProt ID: P12081
• Chromosome Location: 5q31.3
• Pathway: Aminoacyl-tRNA biosynthesis; Gene Expression
• Function: ATP binding; histidine-tRNA ligase activity; histidine-tRNA ligase activity; nucleotide binding