Recombinant Human HLA-A0201 gp100 (YLEPGPVTA) complex protein, His-Avi-tagged

• Cat.No.: HLA-A-732H
• Product Overview: Recombinant Human HLA-A*0201 gp100 (YLEPGPVTA) complex Protein is expressed from mammalian with a polyHis and avi tag at the C-terminal. It contains Gly 25 - Thr 305 (HLA-A*0201),Ile21-Met119 (B2M) and YLEPGPVTA peptide design.

Specification

• Description: Glycoprotein 100, gp100 or Melanocyte protein PMEL is 661 amino acids long and is a type I transmembrane glycoprotein enriched in melanosomes, which are the melanin-producing organelles in melanocytes. This protein is involved in melanosome maturation. The Human HLA-A*0201 gp100 (YLEPGPVTA) complex Protein is a complex of HLA-A*0201 of the MHC Class I, B2M and YLEPGPVTA peptide of the gp100.
• Source: HEK293
• Species: Human
• Tag: His-Avi
• Form: Supplied as 0.22um filtered solution in PBS (pH 7.4).
• Molecular Mass: The protein has a predicted MW of 50.4 kDa.Due to glycosylation, the protein migrates to 52-62 KDa based on Bis-Tris PAGE result.
• Protein length: It contains Gly 25 - Thr 305 (HLA-A*0201),Ile21-Met119 (B2M) and YLEPGPVTA peptide design.
• Endotoxin: Less than 1EU per ug by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
• Storage: The product should be stored at -70°C. Please do not repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: HLA-A major histocompatibility complex, class I, A [ Homo sapiens ]
• Official Symbol: HLA-A
• Synonyms: HLA0201, MHC I,gp100,Glycoprotein 100,ME20-M,Melanocytes lineage-specific antigen GP100,P100
• Gene ID: 3105
• mRNA Refseq: NM_001242758
• Protein Refseq: NP_001229687
• UniProt ID: P01891