Recombinant Human HLA-A protein, His-tagged

• Cat.No.: HLA-A-3396H
• Product Overview: Recombinant Human HLA-A protein(28-303 aa), fused to His tag, was expressed in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: His
• Form: The purified protein was Lyophilized from sterile PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH7.4). 5 % trehalose and 5 % mannitol are added as protectant before lyophilization. The elution buffer contain 300mM imidazole.
• Protein length: 28-303 aa
• AA Sequence: SMRYFFTSVSRPGRGEPRFIAVGYV DDTQFVRFDSDAASQKMEPRAPWIE QEGPEYWDQETRNMKAHSQTDRANL GTLRGYYNQSEDGSHTIQIMYGCDV GPDGRFLRGYRQDAYDGKDYIALNE DLRSWTAADMAAQITKRKWEAVHAA EQRRVYLEGRCVDGLRRYLENGKET LQRTDPPKTHMTHHPISDHEATLRC WALGFYPAEITLTWQRDGEDQTQDT ELVETRPAGDGTFQKWAAVVVPSGE EQRYTCHVQHEGLPKPLTLRWELSS Q
• Purity: 90%, by SDS-PAGE with Coomassie Brilliant Blue staining.
• Storage: Short-term storage: Store at 2-8°C for (1-2 weeks).
  Long-term storage: Aliquot and store at -20°C to -80°C for up to 3 months, buffer containing 50% glycerol is recommended for reconstitution. Avoid repeat freeze-thaw cycles.
• Reconstitution: Reconstitute at 0.25 µg/μl in 200 μl sterile water for short-term storage.
  Reconstitution with 200 μl 50% glycerol solution is recommended for longer term storage.

Gene Information

• Gene Name: HLA-A major histocompatibility complex, class I, A [ Homo sapiens ]
• Official Symbol: HLA-A
• Synonyms: HLA-A; major histocompatibility complex, class I, A; HLA class I histocompatibility antigen, A-1 alpha chain; antigen presenting molecule; leukocyte antigen class I-A; MHC class I antigen HLA-A heavy chain; HLAA; FLJ26655
• Gene ID: 3105
• mRNA Refseq: NM_001242758
• Protein Refseq: NP_001229687
• UniProt ID: P01891