Recombinant Human HLA-B Protein, Myc/DDK-tagged, C13 and N15-labeled

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Recombinant Human HLA-B Protein, Myc/DDK-tagged, C13 and N15-labeled

HLA-B Related Products

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Cat.No. : HLA-B-1894H
Product Overview : HLA MS Standard C13 and N15-labeled recombinant protein (NP_005505) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.
Description : HLA-B belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exon 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-B alleles have been described.
Source : HEK293
Species : Human
Tag : Myc/DDK
Molecular Mass : 40.3 kDa
AA Sequence : MLVMAPRTVL LLLSAALALT ETWAGSHSMR YFDTAMSRPG RGEPRFISVG YVDDTQFVRF DSDAASPREE PRAPWIEQEG PEYWDRNTQI FKTNTQTDRE SLRNLRGYYN QSEAGSHTLQ SMYGCDVGPD GRLLRGHNQY AYDGKDYIAL NEDLRSWTAA DTAAQITQRK WEAARVAEQD RAYLEGTCVE WLRRYLENGK DTLERADPPK THVTHHPISD HEATLRCWAL GFYPAEITLT WQRDGEDQTQ DTELVETRPA GDRTFQKWAA VVVPSGEEQR YTCHVQHEGL PKPLTLRWEP SSQSTVPIVG IVAGLAVLAV VVIGAVVAAV MCRRKSSGGK GGSYSQAACS DSAQGSDVSL TATRTRPLEQ KLISEEDLAA NDILDYKDDD DKV
Purity : > 80% as determined by SDS-PAGE and Coomassie blue staining
Stability : Stable for 3 months from receipt of products under proper storage and handling conditions.
Storage : Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
Concentration : 50 μg/mL as determined by BCA
Storage Buffer : 100 mM glycine, 25 mM Tris-HCl, pH 7.3.
SDS-PAGE :

Gene Name : HLA-B major histocompatibility complex, class I, B [ Homo sapiens (human) ]
Official Symbol : HLA-B
Synonyms : HLA-B; major histocompatibility complex, class I, B; ankylosing spondylitis, AS; lymphocyte antigen; MHC class I antigen SHCHA; MHC Class I HLA heavy chain; MHC class I antigen GN00104; leukocyte antigen class I-B; MHC HLA-B cell surface glycoprotein; MHC HLA-B transmembrane glycoprotein; MHC class I antigen HLA-B heavy chain; HLA class I histocompatibility antigen, B alpha chain; AS; HLAB; SPDA1; MGC111087;
Gene ID : 3106
mRNA Refseq : NM_005514
Protein Refseq : NP_005505
MIM : 142830
UniProt ID : P01889

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