Recombinant Human HLA-DQA1 Protein, Myc/DDK-tagged, C13 and N15-labeled

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Recombinant Human HLA-DQA1 Protein, Myc/DDK-tagged, C13 and N15-labeled

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Cat.No. : HLA-DQA1-5284H
Product Overview : HLA MS Standard C13 and N15-labeled recombinant protein (NP_002113) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.
Description : HLA-DQA1 belongs to the HLA class II alpha chain paralogues. The class II molecule is a heterodimer consisting of an alpha (DQA) and a beta chain (DQB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B Lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa. It is encoded by 5 exons; exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. Within the DQ molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to four different molecules. Typing for these polymorphisms is routinely done for bone marrow transplantation.
Source : HEK293
Species : Human
Tag : Myc/DDK
Molecular Mass : 27.8 kDa
AA Sequence : MILNKALLLG ALALTTVMSP CGGEDIVADH VASCGVNLYQ FYGPSGQFTH EFDGDEQFYV DLEKKETAWR WPEFSKFGGF DPQGALRNMA VAKHNLNIMI KRYNSTAATN EVPEVTVFSK SPVTLGQPNT LICLDNIFPP VVNITWLSNG HAVTEGVSET SFLSKSDHSF FKISYLTFLP SADEIYDCKV EHWGLDQPLL KHWEPEIPAP MSELTETVVC ALGLSVGLVG IVVGTVFIIQ GLRSVGASRH QGPLTRTRPL EQKLISEEDL AANDILDYKD DDDKV
Purity : > 80% as determined by SDS-PAGE and Coomassie blue staining
Stability : Stable for 3 months from receipt of products under proper storage and handling conditions.
Storage : Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
Concentration : 50 μg/mL as determined by BCA
Storage Buffer : 100 mM glycine, 25 mM Tris-HCl, pH 7.3.
SDS-PAGE :

Gene Name : HLA-DQA1 major histocompatibility complex, class II, DQ alpha 1 [ Homo sapiens (human) ]
Official Symbol : HLA-DQA1
Synonyms : HLA-DQA1; major histocompatibility complex, class II, DQ alpha 1; HLA DQA; HLA class II histocompatibility antigen, DQ alpha 1 chain; CELIAC1; HLA-DCA; DC-alpha; DC-1 alpha chain; MHC HLA-DQ alpha; MHC class II DQA1; MHC class II antigen; leucocyte antigen DQA1; MHC class II HLA-DQ-alpha-1; leukocyte antigen alpha chain; MHC class II surface glycoprotein; MHC class II HLA-D alpha glycoprotein; HLA class II histocompatibility antigen, DQ(W3) alpha chain; CD; GSE; DQ-A1; HLA-DQA; FLJ27088; FLJ27328; MGC149527;
Gene ID : 3117
mRNA Refseq : NM_002122
Protein Refseq : NP_002113
MIM : 146880
UniProt ID : P01909

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