Recombinant Human HLA-F protein, His-tagged

• Cat.No.: HLA-F-3439H
• Product Overview: Recombinant Human HLA-F protein(21-298 aa), fused to His tag, was expressed in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: His
• Form: The purified protein was Lyophilized from sterile PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH7.4). 5 % trehalose and 5 % mannitol are added as protectant before lyophilization. The elution buffer contain 300mM imidazole.
• Protein length: 21-298 aa
• AA Sequence: AGSHSLRYFSTAVSRPGRGEPRYIA VEYVDDTQFLRFDSDAAIPRMEPRE PWVEQEGPQYWEWTTGYAKANAQTD RVALRNLLRRYNQSEAGSHTLQGMN GCDMGPDGRLLRGYHQHAYDGKDYI SLNEDLRSWTAADTVAQITQRFYEA EEYAEEFRTYLEGECLELLRRYLEN GKETLQRADPPKAHVAHHPISDHEA TLRCWALGFYPAEITLTWQRDGEEQ TQDTELVETRPAGDGTFQKWAAVVV PSGEEQRYTCHVQHEGLPQPLILRW EQS
• Purity: 85%, by SDS-PAGE with Coomassie Brilliant Blue staining.
• Storage: Short-term storage: Store at 2-8°C for (1-2 weeks).
  Long-term storage: Aliquot and store at -20°C to -80°C for up to 3 months, buffer containing 50% glycerol is recommended for reconstitution. Avoid repeat freeze-thaw cycles.
• Reconstitution: Reconstitute at 0.25 µg/μl in 200 μl sterile water for short-term storage.
  Reconstitution with 200 μl 50% glycerol solution is recommended for longer term storage.

Gene Information

• Gene Name: HLA-F major histocompatibility complex, class I, F [ Homo sapiens ]
• Official Symbol: HLA-F
• Synonyms: HLA-F; major histocompatibility complex, class I, F; HLA class I histocompatibility antigen, alpha chain F; HLA F antigen; leukocyte antigen F; HLA class I molecule; MHC class Ib antigen; MHC class I antigen F; HLAF; CDA12; HLA-5.4; HLA-CDA12
• Gene ID: 3134
• mRNA Refseq: NM_001098478
• Protein Refseq: NP_001091948
• MIM: 143110
• UniProt ID: P30511