Recombinant Human HMGCS2 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: HMGCS2-3611H
• Product Overview: HMGCS2 MS Standard C13 and N15-labeled recombinant protein (NP_005509) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: The protein encoded by this gene belongs to the HMG-CoA synthase family. It is a mitochondrial enzyme that catalyzes the first reaction of ketogenesis, a metabolic pathway that provides lipid-derived energy for various organs during times of carbohydrate deprivation, such as fasting. Mutations in this gene are associated with HMG-CoA synthase deficiency. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 56.6 kDa
• AA Sequence: MQRLLTPVKRILQLTRAVQETSLTP ARLLPVAHQRFSTASAVPLAKTDTW PKDVGILALEVYFPAQYVDQTDLEK YNNVEAGKYTVGLGQTRMGFCSVQE DINSLCLTVVQRLMERIQLPWDSVG RLEVGTETIIDKSKAVKTVLMELFQ DSGNTDIEGIDTTNACYGGTASLFN AANWMESSSWDGRYAMVVCGDIAVY PSGNARPTGGAGAVAMLIGPKAPLA LERGLRGTHMENVYDFYKPNLASEY PIVDGKLSIQCYLRALDRCYTSYRK KIQNQWKQAGSDRPFTLDDLQYMIF HTPFCKMVQKSLARLMFNDFLSASS DTQTSLYKGLEAFGGLKLEDTYTNK DLDKALLKASQDMFDKKTKASLYLS THNGNMYTSSLYGCLASLLSHHSAQ ELAGSRIGAFSYGSGLAASFFSFRV SQDAAPGSPLDKLVSSTSDLPKRLA SRKCVSPEEFTEIMNQREQFYHKVN FSPPGDTNSLFPGTWYLERVDEQHR RKYARRPVTRTRPLEQKLISEEDLA ANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: HMGCS2 3-hydroxy-3-methylglutaryl-CoA synthase 2 [ Homo sapiens (human) ]
• Official Symbol: HMGCS2
• Synonyms: HMGCS2; 3-hydroxy-3-methylglutaryl-CoA synthase 2 (mitochondrial); 3 hydroxy 3 methylglutaryl Coenzyme A synthase 2 (mitochondrial); hydroxymethylglutaryl-CoA synthase, mitochondrial; HMG-CoA synthase; 3-hydroxy-3-methylglutaryl-Coenzyme A synthase 2 (mitochondrial)
• Gene ID: 3158
• mRNA Refseq: NM_005518
• Protein Refseq: NP_005509
• MIM: 600234
• UniProt ID: P54868