Active Recombinant Human HMOX1 protein, His-tagged

• Cat.No.: HMOX1-138H
• Product Overview: Recombinant Human HMOX1(Met1-Thr261) fused with His tag at C-terminal was expressed in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: His
• Predicted N Terminal: Met1
• Form: Supplied as a 0.2 μm filtered solution in Tris, NaCl and Glycerol.
• Bio-activity: Measured by its ability to oxidize hemin to biliverdin. The specific activity is >5 pmol/min/μg, as measured under the described conditions.
• Molecular Mass: Predicted Molecular Mass: 31 kDa;SDS-PAGE: 29-31 kDa, reducing conditions.
• Endotoxin: <1.0 EU per 1 μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane
• Storage: Avoid repeated freeze-thaw cycles.6 months from date of receipt, -20 to -70 centigrade as supplied.3 months, -20 to -70 centigrade under sterile conditions after opening.

Gene Information

• Gene Name: HMOX1 heme oxygenase (decycling) 1 [ Homo sapiens ]
• Official Symbol: HMOX1
• Synonyms: HMOX1; heme oxygenase (decycling) 1; heme oxygenase 1; bK286B10; HO 1; heat shock protein, 32-kD; HO-1; HSP32
• Gene ID: 3162
• mRNA Refseq: NM_002133
• Protein Refseq: NP_002124
• MIM: 141250
• UniProt ID: P09601
• Chromosome Location: 22q12
• Pathway: HIF-1-alpha transcription factor network, organism-specific biosystem; Heme degradation, organism-specific biosystem; Iron uptake and transport, organism-specific biosystem; Keap1-Nrf2 Pathway, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of porphyrins, organism-specific biosystem; Mineral absorption, organism-specific biosystem
• Function: enzyme binding; heme binding; heme oxygenase (decyclizing) activity; heme oxygenase (decyclizing) activity; metal ion binding; oxidoreductase activity; phospholipase D activity; protein homodimerization activity; signal transducer activity