Recombinant Human HPRT1, His-tagged

• Cat.No.: HPRT1-13924H
• Product Overview: Recombinant Human HPRT1 protein, fused to His-tag, was expressed in E.coli and purified by Ni-sepharose.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-218a.a.
• Description: The protein encoded by this gene is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. This enzyme plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutations in this gene result in Lesch-Nyhan syndrome or gout.
• Source: E.coli
• Species: Human
• Tag: His
• Storage: The protein is stored in PBS buffer at -20℃. Avoid repeated freezing and thawing cycles.
• Storage Buffer: 1M PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH8. ) added with 300mM Imidazole and 0.7% Sarcosyl, 15%glycerol.

Gene Information

• Gene Name: HPRT1 hypoxanthine phosphoribosyltransferase 1 [ Homo sapiens ]
• Official Symbol: HPRT1
• Synonyms: HPRT1; hypoxanthine phosphoribosyltransferase 1; HPRT; hypoxanthine-guanine phosphoribosyltransferase; HGPRT; Lesch Nyhan syndrome; HGPRTase
• Gene ID: 3251
• mRNA Refseq: NM_000194
• Protein Refseq: NP_000185
• MIM: 308000
• UniProt ID: P00492
• Chromosome Location: Xq26.2
• Pathway: Drug metabolism - other enzymes, organism-specific biosystem; Drug metabolism - other enzymes, conserved biosystem; Metabolic pathways, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of nucleotides, organism-specific biosystem; Nucleotide Metabolism, organism-specific biosystem; Purine metabolism, organism-specific biosystem
• Function: guanine phosphoribosyltransferase activity; hypoxanthine phosphoribosyltransferase activity; hypoxanthine phosphoribosyltransferase activity; magnesium ion binding; nucleotide binding; NOT phosphoglucomutase activity; protein binding; protein homodimeriza