Recombinant Human HSP70 Protein, His-tagged

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Recombinant Human HSP70 Protein, His-tagged

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Cat.No. : HSPA1A-426H
Product Overview : Recombinant Human HSPA1A (NP_005336.3) (Ala2-Asp641) was expressed with a polyhistidine tag at the N-terminus.
Description : HSPA1A is a member of the Hsp70 protein family. The 70 kilodalton heat sh centigradek proteins (Hsp70s) are a family of ubiquitously expressed heat sh centigradek proteins. HSP are abundant and conserved proteins present in all cells. Upon temperature sh centigradek or other stress stimuli, HSP are synthesized intracellularly, which may protect cells from protein denaturation or from death. Extracellularly, HSP can serve a cytokine function to initiate both innate and adaptive immunity through activation of APC. HSP serves also a chaperone function and facilitates presentation of antigen peptide to T cells. Molecular chaperones of the Hsp70 family have diverse functions in cells. They assist the folding of newly synthesized and stress-denatured proteins, as well as the import of proteins into organelles, and the diss centigradeiation of aggregated proteins. The well-conserved Hsp70 chaperones are ATP dependent: binding and hydrolysis of ATP regulates their interactions with unfolded polypeptide substrates, and ATPase cycling is necessary for their function. All cellular functions of Hsp70 chaperones use the same mechanism of ATP-driven polypeptide binding and release.
Source : HEK293
Species : Human
Tag : His
Predicted N Terminal : Met
Form : Lyophilized from sterile PBS, pH 7.4. Normally 5 %- 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Molecular Mass : The recombinant human HSPA1A consists of 651 amino acids and predicts a molecular mass of 71.4 kDa.
Protein length : Ala2-Asp641
Endotoxin : < 1.0 EU per μg protein as determined by the LAL method.
Purity : > 95 % as determined by SDS-PAGE.
Storage : Samples are stable for up to twelve months from date of receipt at-70 centigrade. Store it under sterile conditions at-20 centigrade to-80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Gene Name : HSPA1A heat shock 70kDa protein 1A [ Homo sapiens ]
Official Symbol : HSPA1A
Synonyms : HSPA1A; heat shock 70kDa protein 1A; heat shock 70kD protein 1A , HSPA1; heat shock 70 kDa protein 1A/1B; HSP70 1; HSP70-1/HSP70-2; HSP70.1/HSP70.2; heat shock 70kD protein 1A; heat shock-induced protein; heat shock 70 kDa protein 1/2; dnaK-type molecular chaperone HSP70-1; HSP72; HSPA1; HSP70I; HSPA1B; HSP70-1; HSP70-1A; FLJ54303; FLJ54370; FLJ54392; FLJ54408; FLJ75127;
Gene ID : 3303
mRNA Refseq : NM_005345
Protein Refseq : NP_005336
MIM : 140550
UniProt ID : P08107

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