| Description : |
ICAM1 (Intercellular Adhesion Molecule-1), also known as CD54, is a transmembrane cell adhesion glycoprotein and a member of the immunoglobulin supergene family. The ICAM sub-family consists of five members, ICAM-1 through ICAM-5, and they vary in their tissue expression and number of Ig-like domains in the extracellular domain (ECD). Full-length ICAM-1 contains five Ig-like domains in the ECD, a single transmembrane domain and short intracellular domain. Alternative splicing of ICAM-1 results in at least six membrane-bound forms and one soluble form (1). The ECD of mature, full-length human ICAM-1 shares 78% and 54% amino acid sequence identity with rat and mouse ICAM-1, respectively. ICAM-1 plays an important role in both innate and adaptive immune responses and is upregulated in endothelial and epithelial cells at sites of inflammation (2, 3). ICAM-1 mediates vascular adhesion and paracellular migration of leukocytes expressing activated LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18) 2, 3). It also binds several non-integrin ligands including CD43/Sialophorin, Fibrinogen, Hyaluronan, rhinoviruses, and Plasmodium falciparum-infected erythrocytes (4-8). Soluble ICAM-1 promotes angiogenesis and serves as an indicator of vascular endothelial cell activation or damage (9). Elevated levels of soluble ICAM-1 are associated with cardiovascular disease, type 2 diabetes, organ transplant dysfunction, oxidant stress, increased abdominal fat mass, hypertension, liver disease, certain malignancies, and cerebral malaria (10-18). More recently, ICAM-1 was shown to be a novel regulator of group 2 innate lymphoid cells (ILC2s) which play a key role in allergic airway inflammation (19). |
| Source : |
HEK293 cells |
| Species : |
Human |
| Tag : |
Fc/Avi |
| Predicted N Terminal : |
Gln28 |
| Form : |
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
| Bio-activity : |
Measured by its binding ability in a functional ELISA. When Human ICAM1 Antibody is immobilized at 0.5 µg/mL (100 µL/well), Biotinylated Recombinant Human ICAM1 Fc Chimera Avi-tag binds with an ED50 of 3-18 ng/mL. Measured by its ability to support the adhesion of HSB2 human peripheral blood acute lymphoblastic leukemia cells. The ED50 for this effect is 0.3-2.7 μg/mL. |
| Molecular Mass : |
95-125 kDa, under reducing conditions |
| Protein length : |
Gln28-Glu480 |
| Endotoxin : |
<0.10 EU per 1 μg of the protein by the LAL method. |
| Purity : |
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. |
| Applications : |
Bioactivity |
| Storage : |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 °C as supplied.1 month, 2 to 8 °C under sterile conditions after reconstitution.3 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Reconstitution : |
Reconstitute at 500 μg/mL in PBS. |
