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Recombinant Human ICOS Protein, Fc-tagged

Cat.No.: ICOS-3244H
Product Overview: Human ICOS / CD278 Protein, Fc Tag, Glu 21 - Phe 141 (Accession # NP_036224) was produced in human 293 cells (HEK293).
Source: HEK293
Species: Human
Tag: Fc
Predicted N Terminal: Glu 21
Form: Lyophilized from sterile 50 mM tris, 100 mM glycine, pH7.5, 10% Trehalose.
Bio-activity: Measured by its binding ability in a functional ELISA against immobilized Human B7-H2, His Tag (0.1μg/well). The linear is 0.07-2.3 ng/mL.
Molecular Mass: Human ICOS, Fc Tag is fused with a human IgG1 Fc tag at the C-terminus, and has a calculated MW of 40.4 kDa. The reducing protein migrates as 45-50 kDa in SDS-PAGE due to glycosylation.
Endotoxin: Less than 1.0 EU per μg by the LAL method.
Purity: 50% human ICOS (Fc Tag) and 50% human IgG1 Fc
Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Gene Name: ICOS inducible T-cell co-stimulator [ Homo sapiens ]
Official Symbol: ICOS
Synonyms: ICOS; inducible T-cell co-stimulator; inducible T-cell costimulator; activation inducible lymphocyte immunomediatory molecule; AILIM; CD278; inducible costimulator; activation-inducible lymphocyte immunomediatory molecule; CVID1; MGC39850;
Gene ID: 29851
mRNA Refseq: NM_012092
Protein Refseq: NP_036224
MIM: 604558
UniProt ID: Q9Y6W8
Chromosome Location: 2q33
Pathway: Adaptive Immune System, organism-specific biosystem; Cell adhesion molecules (CAMs), organism-specific biosystem; Cell adhesion molecules (CAMs), conserved biosystem; Costimulation by the CD28 family, organism-specific biosystem; Immune System, organism-specific biosystem; Intestinal immune network for IgA production, organism-specific biosystem; Intestinal immune network for IgA production, conserved biosystem;

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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