Active Recombinant Human ICOS(Q50A) Protein, Fc-tagged

• Cat.No.: ICOS-4422H
• Product Overview: Active Recombinant Human ICOS(Q50A) Protein(Q9Y6W8-1)(Glu21-Phe141) is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: 21-141 aa
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
• Bio-activity: Immobilized Human ICOS (Q50A), hFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-ICOS Antibody, hFc Avi Tag with the EC50 of 91.0ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 40.45 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 1 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
• Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: ICOS inducible T-cell co-stimulator [ Homo sapiens ]
• Official Symbol: ICOS
• Synonyms: ICOS; inducible T-cell co-stimulator; inducible T-cell costimulator; activation inducible lymphocyte immunomediatory molecule; AILIM; CD278; inducible costimulator; activation-inducible lymphocyte immunomediatory molecule; CVID1; MGC39850
• Gene ID: 29851
• mRNA Refseq: NM_012092
• Protein Refseq: NP_036224
• MIM: 604558
• UniProt ID: Q9Y6W8