Recombinant Rhesus ICOS protein(Met1-Lys140), hFc&Avi-tagged, Biotinylated

• Cat.No.: ICOS-1611R
• Product Overview: Recombinant Rhesus ICOS (NP_001253918.1) (Met1-Lys140) was expressed in HEK293 with a C-terminal Fc region of Human IgG1 tag followed by an AVI tag. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.

Specification

• Source: HEK293
• Species: Rhesus
• Tag: C-hFc&Avi
• Protein length: Met1-Lys140
• Conjugation/Label: Biotin
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Immobilized Recombinant Cynomolgus ICOS Ligand / B7-H2 Protein (Fc Tag) at 2 μg/ml (100 μl/well) can bind Recombinant Rhesus ICOS Protein (Fc & AVI Tag), Biotinylated, The EC50 is 4-12 ng/mL.
• Molecular Mass: The recombinant rhesus ICOS consists of 374 amino acids and predicts a molecular mass of 42.2 kDa.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 90 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.