Recombinant Human IFT57 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: IFT57-965H
• Product Overview: IFT57 MS Standard C13 and N15-labeled recombinant protein (NP_060480) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: Required for the formation of cilia. Plays an indirect role in sonic hedgehog signaling, cilia being required for all activity of the hedgehog pathway. Has pro-apoptotic function via its interaction with HIP1, leading to recruit caspase-8 (CASP8) and trigger apoptosis. Has the ability to bind DNA sequence motif 5'-AAAGACATG-3' present in the promoter of caspase genes such as CASP1, CASP8 and CASP10, suggesting that it may act as a transcription regulator; however the relevance of such function remains unclear.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 49.1 kDa
• AA Sequence: MTAALAVVTTSGLEDGVPRSRGEGT GEVVLERGPGAAYHMFVVMEDLVEK LKLLRYEEEFLRKSNLKAPSRHYFA LPTNPGEQFYMFCTLAAWLINKAGR PFEQPQEYDDPNATISNILSELRSF GRTADFPPSKLKSGYGEHVCYVLDC FAEEALKYIGFTWKRPIYPVEELEE ESVAEDDAELTLNKVDEEFVEEETD NEENFIDLNVLKAQTYHLDMNETAK QEDILESTTDAAEWSLEVERVLPQL KVTIRTDNKDWRIHVDQMHQHRSGI ESALKETKGFLDKLHNEITRTLEKI SSREKYINNQLENLVQEYRAAQAQL SEAKERYQQGNGGVTERTRLLSEVM EELEKVKQEMEEKGSSMTDGAPLVK IKQSLTKLKQETVEMDIRIGIVEHT LLQSKLKEKSNMTRNMHATVIPEPA TGFYTRTRPLEQKLISEEDLAANDI LDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: IFT57 intraflagellar transport 57 [ Homo sapiens (human) ]
• Official Symbol: IFT57
• Synonyms: IFT57; intraflagellar transport 57 homolog (Chlamydomonas); ESRRBL1, estrogen related receptor beta like 1; intraflagellar transport protein 57 homolog; FLJ10147; HIPPI; MHS4R2; HIP1 protein interactor; HIP1-interacting protein; dermal papilla-derived protein 8; estrogen-related receptor beta like 1; estrogen-related receptor beta-like protein 1; huntingtin interacting protein-1 interacting protein; ESRRBL1
• Gene ID: 55081
• mRNA Refseq: NM_018010
• Protein Refseq: NP_060480
• MIM: 606621
• UniProt ID: Q9NWB7