Recombinant Human IGF1R Protein, His/GST-tagged, Alexa Fluor 647 conjugated

• Cat.No.: IGF1R-50HAF647
• Product Overview: Alexa Fluor 647 conjugated recombinant human IGF1R (NP_000866.1) cytoplasmic domain (Met 954-Cys 1367), fused with the N-terminal polyhistidine-tagged GST tag, was produced in Baculovirus-Insect cells.

Specification

• Source: Insect Cells
• Species: Human
• Tag: His&GST
• Form: Lyophilized
• Molecular Mass: The recombinant human IGF1R (aa 954-1367) /GST chimera consists of 651 amino acids and has a calculated molecular mass of 74.6 kDa. It migrates as an approximately 70 kDa band in SDS-PAGE under reducing conditions.
• Protein length: 651
• Endotoxin: < 1.0 EU/ μg of the protein as determined by the LAL method.
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 647 via amines
  Excitation = 650 nm
  Emission = 668 nm
• Stability: Samples are stable for up to 12 months from date of receipt at -70 centigrade.
• Storage: Store it under sterile conditions at -20 to -70 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Lyophilized from 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, pH 7.4, 20% glycerol, 0.3 mM DTT
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: IGF1R insulin-like growth factor 1 receptor [ Homo sapiens ]
• Official Symbol: IGF1R
• Gene ID: 3480
• mRNA Refseq: NM_000875
• Protein Refseq: NP_000866
• MIM: 147370
• UniProt ID: P08069

Reviews

07/08/2022

The product's exceptional purity facilitated precise quantification, ensuring data consistency.

06/14/2019

The product consistently delivered robust and dependable results, affirming its efficacy in our experiments.

04/29/2018

The product's consistent performance bolstered the dependability of our research conclusions.

Q&As

In which cellular compartments is IGF1R predominantly localized?

IGF1R is predominantly localized on the cell membrane, where it acts as a receptor for insulin-like growth factors (IGFs) and insulin.

Are there specific downstream signaling pathways activated by IGF1R, and how do they influence cell proliferation and survival?

Downstream signaling pathways activated by IGF1R include the Ras/MAPK pathway and PI3K/Akt pathway, contributing to cell growth and survival.

How does IGF1R interact with insulin-like growth factors (IGFs) and insulin, and what is its role in growth regulation?

IGF1R interacts with IGFs and insulin, triggering downstream signaling pathways such as the PI3K/Akt pathway, influencing cell proliferation and survival.

Are there therapeutic implications or potential targets related to IGF1R in diseases involving growth signaling?

There are potential therapeutic implications related to IGF1R in diseases involving growth signaling, making it a target for drug development.

What diseases or conditions are associated with dysregulation or abnormal expression of IGF1R?

Dysregulation or abnormal expression of IGF1R is associated with conditions such as cancer and developmental disorders.

What is the primary function of Insulin-like growth factor 1 receptor (IGF1R) in cellular processes and growth signaling?

Insulin-like growth factor 1 receptor (IGF1R) functions in cellular processes, primarily playing a role in growth signaling.

How does IGF1R contribute to the regulation of tissue development, particularly in the context of growth and differentiation?

IGF1R contributes to the regulation of tissue development by influencing cell growth, differentiation, and survival.