Recombinant Human IL10RA, His-tagged

• Cat.No.: IL10RA-54H
• Product Overview: Recombinant human IL10RA, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Specification

• Species: Human
• Source: Insect Cells
• Tag: His
• Description: IL10RA, also known as Interleukin-10 receptor subunit alpha, is structurally related to interferon receptors. It has been shown to mediate the immunosuppressive signal of interleukin 10, and thus inhibits the synthesis of proinflammatory cytokines. This receptor is reported to promote survival of progenitor myeloid cells through the insulin receptor substrate-2/PI 3-kinase/AKT pathway. Activation of this receptor leads to tyrosine phosphorylation of JAK1 and TYK2 kinases. It has been shown to interact with Interleukin 10 and Janus kinase 1.
• Form: Liquid. In phosphate buffered saline (pH 7.4) containing 10% glycerol.
• Molecular Mass: 25.2kDa (220aa) 228-40kDa (SDS-PAGE under reducing conditions.)
• AA Sequence: HGTELPSPPS VWFEAEFFHH ILHWTPIPNQ SESTCYEVAL LRYGIESWNS ISNCSQTLSY DLTAVTLDLY HSNGYRARVR AVDGSRHSNW TVTNTRFSVD EVTLTVGSVN LEIHNGFILG KIQLPRPKMA PANDTYESIF SHFREYEIAI RKVPGNFTFT HKKVKHENFS LLTSGEVGEF CVQVKPSVAS RSNKGMWSKE ECISLTRQYF TVTNHHHHHH
• Endotoxin: < 1.0 eu per 1 microgram of protein (determined by lal
• Purity: >85% by SDS - PAGE.
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.2mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: IL10RA interleukin 10 receptor, alpha [ Homo sapiens ]
• Official Symbol: IL10RA
• Synonyms: IL10RA; interleukin 10 receptor, alpha; IL10R; interleukin-10 receptor subunit alpha; CD210; CD210a; CDW210A; HIL 10R; IL-10RA; IL-10R subunit 1; IL-10R subunit alpha; IL-10 receptor subunit alpha; interleukin-10 receptor subunit 1; interleukin-10 receptor alpha chain; HIL-10R; IL-10R1
• Gene ID: 3587
• mRNA Refseq: NM_001558
• Protein Refseq: NP_001549
• MIM: 146933
• UniProt ID: Q13651
• Chromosome Location: 11q23
• Pathway: Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Jak-STAT signaling pathway, organism-specific biosystem; Jak-STAT signaling pathway, conserved biosystem; Toxoplasmosis, organism-specific biosystem; Toxoplasmosis, conserved biosystem; Tuberculosis, organism-specific biosystem
• Function: interleukin-10 receptor activity; protein binding; receptor activity; signal transducer activity