Recombinant Human IL10RB Protein, Met20-Ser220, C-Fc tagged

• Cat.No.: IL10RB-202H
• Product Overview: Recombinant Human IL-10 R beta Protein (Met20-Ser220) with Human IgG1 (Pro100-Lys330) Fc tag at C-terminus was expressed in Chinese Hamster Ovary cell line.

Specification

• Description: The protein encoded by this gene belongs to the cytokine receptor family. It is an accessory chain essential for the active interleukin 10 receptor complex. Coexpression of this and IL10RA proteins has been shown to be required for IL10-induced signal transduction. This gene and three other interferon receptor genes, IFAR2, IFNAR1, and IFNGR2, form a class II cytokine receptor gene cluster located in a small region on chromosome 21.
• Source: CHO
• Species: Human
• Tag: Fc
• Protein length: Met20-Ser220
• Form: Disulfide-linked homodimer
• Bio-activity: Measured by its binding ability in a functional ELISA. Recombinant Human IL-10 R beta Fc Chimera binds Recombinant Human IL-28 R alpha/IFN-lambda R1 Fc Chimera in the presence of Recombinant Human IL-28B/IFN-lambda 3 with an ED50 of 0.250-3.00 μg/mL.
• Molecular Mass: Predicted Molecular Mass: 50 kDa
  SDS-PAGE: 72-82 kDa, under reducing conditions.
• Endotoxin: < 0.10 EU/μg of the protein by the LAL method.
• Purity: > 95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
• N-terminal Sequence Analysis: Met20
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Shipping: The product is shipped at ambient temperature.
• Reconstitution: Reconstitute at 500 μg/mL in PBS.
• References: 1. Commins, S. et al. (2008) J. Allergy Clin. Immunol. 121:1108.
  2. Pestka, S. et al. (2004) Annu. Rev. Immunol. 22:929.
  3. Kotenko, S.V. et al. (1997) EMBO J. 16:5894.

Gene Information

• Gene Name: IL10RB interleukin 10 receptor, beta [ Homo sapiens (human) ]
• Official Symbol: IL10RB
• Synonyms: IL10RB; interleukin 10 receptor, beta; CRFB4, D21S58, D21S66; interleukin-10 receptor subunit beta; CDW210B; CRF2 4; IL 10R2; IL-10RB; IL-10R subunit 2; IL-10R subunit beta; IL-10 receptor subunit beta; cytokine receptor class-II CRF2-4; interleukin-10 receptor subunit 2; cytokine receptor class-II member 4; cytokine receptor family 2 member 4; cytokine receptor family II, member 4; CRFB4; CRF2-4; D21S58; D21S66; IL-10R2
• Gene ID: 3588
• mRNA Refseq: NM_000628
• Protein Refseq: NP_000619
• MIM: 123889
• UniProt ID: Q08334

Reviews

04/28/2022

Reliable in co-immunoprecipitation.

06/13/2020

High specificity.

12/04/2019

Effective in signaling pathways.

Q&As

Can you explain the role of mass spectrometry-based interactomics in identifying IL10RB-interacting proteins?

Mass spectrometry-based interactomics identifies proteins interacting with IL10RB.

How does NMR spectroscopy help in understanding dynamic interactions and structural changes in IL10RB upon ligand binding?

NMR spectroscopy helps understand dynamic interactions and structural shifts in IL10RB upon binding.

How do proximity labeling and quantitative proteomics reveal changes in IL10RB's protein network upon IL-10 stimulation?

Proximity labeling and quantitative proteomics show IL10RB's changing protein network with IL-10.

How do techniques like surface plasmon resonance and isothermal titration calorimetry contribute to studying IL10RB-IL-10 binding kinetics?

Techniques like surface plasmon resonance and isothermal titration calorimetry measure IL10RB-IL-10 binding strength and kinetics.

What insights do flow cytometry and single-cell transcriptomics provide about IL10RB's expression across cell types and immune subsets?

Flow cytometry and single-cell transcriptomics reveal IL10RB's expression diversity in cells and immune subsets.

Could you explain how mutagenesis and alanine scanning identify critical amino acids in IL10RB's binding to IL-10?

Mutagenesis and alanine scanning pinpoint amino acids essential for IL10RB's binding to IL-10.

How are CRISPR-edited cell lines and gene knockout models utilized to explore IL10RB's downstream signaling pathways after IL-10 binding?

CRISPR-edited cells and knockout models explore IL10RB's signaling after IL-10 binding.

What methods are used to reveal structural changes in IL10RB upon interaction with IL-10, such as X-ray crystallography and cryo-electron microscopy?

X-ray crystallography and cryo-electron microscopy provide structural insights into IL10RB's changes upon IL-10 interaction.

What does genome-wide CRISPR screening reveal about genes and pathways influencing IL10RB expression and function?

Genome-wide CRISPR screening uncovers genes affecting IL10RB expression and function.

What experimental approaches, like co-immunoprecipitation and FRET, are used to study protein complexes involving IL10RB and IL-10?

Co-immunoprecipitation and FRET study protein complexes involving IL10RB and IL-10.