Recombinant Human IL12 p40 (Ile23-Ser328) and p35 (Arg23-Ser219) Protein

• Cat.No.: IL12-47H
• Product Overview: Recombinant human IL-12 p40 (Ile23-Ser328) and human IL-12 p35 (Arg23-Ser219) without tag was expressed in Spodoptera frugiperda. Disulfide-linked heterodimer.

Specification

• Species: Human
• Source: Insect cells
• Protein Length: p40 (Ile23-Ser328) and p35 (Arg23-Ser219)
• Description: Interleukin 12, also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a pleiotropic cytokine originally identified in the medium of activated human B lymphoblastoid cell lines. The p40 subunit of IL-12 has been shown to have extensive amino acid sequence homology to the extracellular domain of the human IL-6 receptor while the p35 subunit shows distant but significant sequence similarity to IL-6, G-CSF, and chicken MGF. These observations have led to the suggestion that IL-12 might have evolved from a cytokine/soluble receptor complex. Human and murine IL-12 share 70% and 60% amino acid sequence homology in their p40 and p35 subunits, respectively. IL-12 apparently shows species specificity with human IL-12 reportedly showing minimal activity in the murine system.
• Bio-activity: Measured in a cell proliferation assay using PHA-stimulated human T lymphoblasts. The ED50 for this effect is 0.01-0.05 ng/mL.
• Molecular Mass: Predicted Molecular Mass: 34.7 kDa (p40) & 22.5 kDa (p35)
  SDS-PAGE: 41 kDa (p40) & 29 kDa (p35), reducing conditions
• Endotoxin: <1.0 EU/μg of the protein by the LAL method.
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• N-terminal Sequence Analysis: Ile23 (p40) & Arg23 (p35)
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Shipping: The product is shipped at ambient temperature.
• Reconstitution: Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
• Full Length: Full L.

Gene Information

• Gene Name: IL12B interleukin 12B [ Homo sapiens (human) ]
• Official Symbol: IL12B
• Synonyms: CLMF p35; CLMF1; Cytotoxic lymphocyte maturation factor 35 kDa subunit; IL-12 subunit p35; IL12; IL-12; IL-12, subunit p35; IL12A; interleukin 12, p35; interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocytematuration factor 1, p35); interleukin-12 alpha chain; interleukin-12 subunit alpha; natural killer cell stimulatory factor 1, 35 kD subunit; NF cell stimulatory factor chain 1; NK cell stimulatory factor chain 1; NKSF1; p35
• Gene ID: 3593
• mRNA Refseq: NM_002187
• Protein Refseq: NP_002178
• MIM: 161561
• UniProt ID: P29460