| Description : |
Interleukin 13 (IL13) is also known as ALRH, BHR1and P600, is a single-chain glycosylated polypeptide, and is a cytokine critical in regulating inflammatory and immune responses. IL13 is secreted by many cell types, but especially by T helper type 2 (Th2) cells. IL-13 induces its effects through a multi-subunit receptor that includes the alpha chain of the IL-4 receptor (IL-4Rα) and at least one of two known IL-13-specific binding chains. The functions of IL-13 overlap considerably with those of IL-4, especially with regard to changes induced on hematopoietic cells, but these effects are probably less important given the more potent role of IL-4. IL-13 induces matrix metalloproteinases (MMPs) as part of a mechanism that protects against excessive allergic inflammation that predisposes to asphyxiation. IL-13 induces many features of allergic lung disease, including airway hyperresponsiveness, goblet cell metaplasia and mucus hypersecretion, which all contribute to airway obstruction. |
| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
Fc |
| Predicted N Terminal : |
Gly |
| Form : |
Lyophilized from 0.22 μm filtered solution in 50 mM Tris,100 mM Glycine, pH7.5, 10% trehalose. |
| Bio-activity : |
Measured by its binding ability in a functional ELISA. Immobilized Human IL-13RA1, His Tag at 10 μg/mL (100 μL/well) can bind Human IL-13, Fc Tag with a linear range of 0.256-10 μg/mL. Measured by its binding ability in a functional ELISA. Immobilized Human IL-13, Fc Tag at 10 μg/mL (100 μL/well) can bind Anti IL-13 MAb, Human IgG4 with a linear range of 0.8-25 ng/mL. |
| Molecular Mass : |
This protein carries a human IgG1 Fc tag at the N-terminus. The protein has a calculated MW of 40.2 kDa. The protein migrates as 45-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
| Protein length : |
Leu 25 - Asn 146 |
| Endotoxin : |
Less than 1.0 EU per μg by the LAL method. |
| Purity : |
>95% as determined by SDS-PAGE. |
| Storage : |
For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. |
| Storage buffer : |
50 mM Tris,100 mM Glycine, pH7.5 |
| Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents. |
| Shipping : |
Powder,RT |
