Active Recombinant Human IL17RB protein, His-tagged

• Cat.No.: IL17RB-2295H
• Product Overview: Active Recombinant Human IL17RB protein(Q9NRM6)(61-200 aa), fused with N-terminal His tag, was expressed in E.coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 61-200 aa
• Form: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Bio-activity: IL17RB (Interleukin-17 receptor B) is a receptor for the proinflammatory cytokines IL17B (Interleukin-17B) and IL17E (Interleukin-17E). This receptor has been shown to mediate the activation of NF-kappaB and the production of IL8 induced by IL17E. Thus a binding ELISA assay was constructed to detect the association of recombinant human IL17RB with recombinant human IL17B. Briefly, IL17RB were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IL17B-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-IL17RB pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of IL17RB with IL17B was shown and this effect was in a dose dependent manner.
• Molecular Mass: 19.4kDa
• Purity: > 80%
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Store at -20°C for 12 months. Aliquot and store at -80°C for 12 months.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: IL17RB interleukin 17 receptor B [ Homo sapiens ]
• Official Symbol: IL17RB
• Synonyms: IL17RB; interleukin 17 receptor B; IL17BR, interleukin 17B receptor; interleukin-17 receptor B; CRL4; EVI27; IL17RH1; IL-17RB; IL-17Rh1; IL-17B receptor; IL-17 receptor B; cytokine receptor CRL4; IL-17 receptor homolog 1; cytokine receptor-like 4; interleukin 17B receptor; interleukin-17B receptor; interleukin 17 receptor homolog 1; IL17BR; MGC5245
• Gene ID: 55540
• mRNA Refseq: NM_018725
• Protein Refseq: NP_061195
• MIM: 605458
• UniProt ID: Q9NRM6