Recombinant Human IL17RC cell lysate
|Product Overview :||Human IL17RC (aa 1-454) derived in Baculovirus-Insect cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls|
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|Source :||Baculovirus-Insect Cells|
|Preparation method :||Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.|
|Lysis buffer :||Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF|
|Quality control Testing :||12.5% SDS-PAGE Stained with Coomassie Blue|
|Recommended Usage :||1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.|
|Stability :||Samples are stable for up to twelve months from date of receipt at -80°C|
|Storage Buffer :||50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF|
|Storage Instruction :||Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.|
|Gene Name :||IL17RC interleukin 17 receptor C [ Homo sapiens ]|
|Official Symbol :||IL17RC|
|Synonyms :||IL17RC; interleukin 17 receptor C; interleukin-17 receptor C; IL17 RL; IL-17RL; IL17Rhom; zcytoR14; IL17F receptor; IL-17 receptor C; interleukin-17 receptor homolog; interleukin-17 receptor-like protein; IL17RL; IL17-RL; FLJ95963; FLJ96005; MGC10763;|
|Gene ID :||84818|
|mRNA Refseq :||NM_001203263|
|Protein Refseq :||NP_001190192|
|UniProt ID :||Q8NAC3|
|Chromosome Location :||3p25.3|
|Function :||receptor activity;|
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Q&As (10)Ask a question
Co-stimulation assays, co-immunoprecipitation, and gene expression analysis reveal IL17RC's interplay with other immune pathways.
Structural studies provide insights into IL17RC's binding interface with ligands, aiding in understanding cytokine-receptor interactions.
In vitro cell migration assays, cytokine profiling, and adoptive transfer experiments investigate IL17RC's impact on immune cell functions.
Animal models, patient-derived cells, and genetic association studies explore IL17RC's involvement in autoimmune disorders.
Conditional knockout models, adoptive transfer experiments, and tissue-specific inflammation assays unveil IL17RC's role in disease contexts.
Biomarker analysis, patient stratification, and correlation studies validate the clinical relevance of IL17RC in human diseases.
Preclinical models, therapeutic antibodies, and disease amelioration studies assess IL17RC as a target for immune-mediated disorders.
Phosphorylation assays, reporter gene assays, and CRISPR screens dissect IL17RC-mediated signaling events and immune outcomes.
Immunofluorescence, flow cytometry, and single-cell RNA sequencing determine IL17RC expression profiles in diverse cell populations.
Perturbation studies, siRNA knockdown, and gene editing differentiate IL17RC's roles in distinct IL17 cytokine pathways.
Customer Reviews (3)Write a review
High purity. -
Suitable for signaling studies. -
Effective in neutrophil activation. -
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