Recombinant Human IL18BP Protein
|Product Overview :||Recombinant Human IL18BP Protein without tag was expressed in CHO.|
- Gene Information
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|Description :||Interleukin-18 binding protein, also known as IL-18BP and tadekinig-alfa, is a secreted glycoprotein that contains an Ig-like C2-type domain. It is expressed in heart, lung, placenta and spleen. IL-18BP functions as an inhibitor of the proinflammatory cytokine IL-18. It binds to IL-18, prevents the binding of IL-18 to its receptor, and thus blocks IL-18-induced IFN-gamma production. The complete Ig domain has been shown to mediate the binding and neutralizing properties. IFN-gamma is able to upregulate the expression of IL-18BP, indicating that IL-18 activity is regulated by a feedback mechanism through IL-18BP.|
|Form :||Sterile Filtered White lyophilized (freeze-dried) powder.|
|Bio-activity :||ED50 < 30 ng/mL, measured in a bioassay using KG-1 cells in the presence of 50 ng/mL Human IL-18.|
|Molecular Mass :||42-44 kDa, observed by reducing SDS-PAGE.|
|AA Sequence :||TPVSQTTTAATASVRSTKDPCPSQP PVFPAAKQCPALEVTWPEVEVPLNG TLSLSCVACSRFPNFSILYWLGNGS FIEHLPGRLWEGSTSRERGSTGTQL CKALVLEQLTPALHSTNFSCVLVDP EQVVQRHVVLAQLWAGLRATLPPTQ EALPSSHSSPQQQG|
|Endotoxin :||< 0.2 EU/μg, determined by LAL method.|
|Purity :||> 95% as analyzed by SDS-PAGE and HPLC.|
|Storage :||Lyophilized recombinant Human IL-18BP remains stable up to 6 months at -80 centigrade from date of receipt. Upon reconstitution, Human IL-18BP should be stable up to 1 week at 4 centigrade or up to 2 months at -20 centigrade.|
|Storage Buffer :||Lyophilized after extensive dialysis against PBS.|
|Reconstitution :||Reconstituted in ddH2O or PBS at 100 μg/mL.|
|Gene Name :||IL18BP interleukin 18 binding protein [ Homo sapiens ]|
|Official Symbol :||IL18BP|
|Synonyms :||IL18BP; interleukin 18 binding protein; interleukin-18-binding protein; IL18BPa; MC51L 53L 54L homolog gene product; IL-18BP; tadekinig-alfa; MC51L-53L-54L homolog gene product;|
|Gene ID :||10068|
|mRNA Refseq :||NM_001039659|
|Protein Refseq :||NP_001034748|
|UniProt ID :||O95998|
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Q&As (10)Ask a question
Genetic association studies, sequencing analyses, and functional investigations explore how genetic variations in IL18BP might influence disease susceptibility and outcomes.
Knockout mice, transgenic models, and imaging techniques provide insights into IL18BP's impact on IL18-related immune responses in vivo.
Promoter studies, siRNA knockdown, and cytokine stimulation assays investigate IL18BP regulation at the transcriptional and post-transcriptional levels.
Luciferase reporter assays, ELISAs, and functional cell assays probe how IL18BP modulates IL18-induced cytokine production and immune cell activation.
Biophysical techniques, site-directed mutagenesis, and receptor binding assays reveal potential allosteric effects on IL18 signaling.
Surface plasmon resonance, isothermal titration calorimetry, and kinetic binding assays quantify IL18BP-IL18 interactions.
RT-qPCR, immunohistochemistry, and tissue microarray analyses determine the expression patterns of IL18BP in different tissues and diseases.
X-ray crystallography, NMR spectroscopy, and mutagenesis studies elucidate the structural basis of IL18BP-IL18 recognition.
Cross-competition assays, co-stimulation studies, and receptor activation assays unveil potential interplay between IL18BP and other cytokine regulators.
Therapeutic antibody trials, in vitro functional screens, and disease model assessments evaluate IL18BP modulation as a potential anti-inflammatory strategy.
Customer Reviews (3)Write a review
Consistent binding affinity. -
High purity. -
Effective in neutralization assays. -
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