Active Recombinant Human IL1R1 protein, His-tagged

• Cat.No.: IL1R1-3147H
• Product Overview: Recombinant Human IL1R1 protein(Asp21-Thr332), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 21-332 aa
• Form: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
• Bio-activity: 1. Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human IL1RA at 5 μg/mL (100 μL/well) can bind Recombinant Human IL1R1 with a linear range of 63-252 ng/mL. 2. Measured by its binding ability in a functional ELISA. Immobilized Human IL-1 beta at 10 μg/mL (100 μL/well) can bind Biotinylated Human IL-1R1 with a linear range of 0.002-1 μg/mL.
• Molecular Mass: 36.70 kDa
• Endotoxin: < 0.1 EU/μg of the protein by LAL method.
• Purity: ≥ 95 % as determined by SDS-PAGE.
• Storage: Store at -20°C.Store the lyophilized protein at -20°C to -80°C up to 1 year from the date of receipt.After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
• Reconstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Gene Information

• Gene Name: IL1R1 interleukin 1 receptor, type I [ Homo sapiens ]
• Official Symbol: IL1R1
• Synonyms: IL1R1; interleukin 1 receptor, type I; IL1R, IL1RA; interleukin-1 receptor type 1; CD121A; D2S1473; IL-1R-1; IL-1RT1; IL-1RT-1; antigen CD121a; interleukin receptor 1; interleukin-1 receptor alpha; interleukin-1 receptor type I; CD121 antigen-like family member A; interleukin 1 receptor alpha, type I; P80; IL1R; IL1RA; IL-1R-alpha
• Gene ID: 3554
• mRNA Refseq: NM_000877
• Protein Refseq: NP_000868
• MIM: 147810
• UniProt ID: P14778