Active Recombinant Mouse IL1R1 Protein, Fc-tagged

• Cat.No.: IL1R1-917M
• Product Overview: Recombinant mouse IL1R1 (P13504) extracellular domain (Met 1-Lys 338) was fused with the Fc region of human IgG1 at the C-terminus.

Specification

• Species: Mouse
• Source: HEK293
• Tag: Fc
• Protein Length: 1-338 a.a.
• Predicted N Terminal: Leu 20
• Form: Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
• Bio-activity: 1. Measured by its binding ability in a functional ELISA. 2. Immobilized recombinant human IL1b at 10 μg/mL (100 μl/well) can mouse IL1R1-Fc with a linear range of 62.5-1000 ng/mL.
• Molecular Mass: The secreted recombinant mouse IL1R1/Fc is a disulfide-linked homodimer. The reduced monomer comprises 560 amino acids and has a calculated molecular mass of 64.3 kDa. As a result of glycosylation, the apparent molecular mass of the recombinant protein is approximately 70 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >92 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: Il1r1 interleukin 1 receptor, type I [ Mus musculus ]
• Official Symbol: IL1R1
• Synonyms: IL1R1; interleukin 1 receptor, type I; interleukin-1 receptor type 1; p80; IL-1R-1; IL-1RT1; IL-1RT-1; IL-1R-alpha; IL-1 receptor alpha chain; interleukin-1 receptor alpha; interleukin-1 receptor type I; CD121 antigen-like family member A; IL-iR; CD121a
• Gene ID: 16177
• mRNA Refseq: NM_001123382
• Protein Refseq: NP_001116854
• Pathway: Amoebiasis, organism-specific biosystem; Amoebiasis, conserved biosystem; Apoptosis, organism-specific biosystem; Apoptosis, conserved biosystem; Apoptosis signaling pathway, organism-specific biosystem; Cytokine-cytokine receptor interaction, organism-sp
• Function: interleukin-1 receptor activity; interleukin-1 receptor activity; interleukin-1, Type I, activating receptor activity; ionotropic glutamate receptor binding; platelet-derived growth factor receptor binding; protease binding; protein binding; receptor acti