Recombinant Human IL1R2 Protein, Fc-tagged

• Cat.No.: IL1R2-307H
• Product Overview: Recombinant human IL1R2 protein with Fc tag was expressed in HEK293.

Specification

• Description: The protein encoded by this gene is a cytokine receptor that belongs to the interleukin 1 receptor family. This protein binds interleukin alpha (IL1A), interleukin beta (IL1B), and interleukin 1 receptor, type I(IL1R1/IL1RA), and acts as a decoy receptor that inhibits the activity of its ligands. Interleukin 4 (IL4) is reported to antagonize the activity of interleukin 1 by inducing the expression and release of this cytokine. This gene and three other genes form a cytokine receptor gene cluster on chromosome 2q12. Alternative splicing results in multiple transcript variants and protein isoforms. Alternative splicing produces both membrane-bound and soluble proteins. A soluble protein is also produced by proteolytic cleavage.
• Source: HEK293
• Species: Human
• Tag: Fc
• Form: Lyophilized
• Molecular Mass: 64 kDa
• Protein length: 398
• AA Sequence: MLRLYVLVMGVSAFTLQPAAHTGAA RSCRFRGRHYKREFRLEGEPVALRC PQVPYWLWASVSPRINLTWHKNDSA RTVPGEEETRMWAQDGALWLLPALQ EDSGTYVCTTRNASYCDKMSIELRV FENTDAFLPFISYPQILTLSTSGVL VCPDLSEFTRDKTDVKIQWYKDSLL LDKDNEKFLSVRGTTHLLVHDVALE DAGYYRCVLTFAHEGQQYNITRSIE LRIKKKKEETIPVIISPLKTISASL GSRLTIPCKVFLGTGTPLTTMLWWT ANDTHIESAYPGGRVTEGPRQEYSE NNENYIEVPLIFDPVTREDLHMDFK CVVHNTLSFQTLRTTVKEASSTFSW GIVLAPLSLAFLVLGGIWMHRRCKH RTGKADGLTVLWPHHQDFQSYPK
• Purity: > 98%
• Applications: WB; ELISA; FACS; FC
• Stability: This bioreagent is stable at 4 centigrade (short-term) and -70 centigrade(long-term). After reconstitution, sample may be stored at 4 centigrade for 2-7 days and below -18 centigrade for future use.
• Storage: At -20 centigrade.
• Concentration: 1 mg/mL
• Storage Buffer: PBS (pH 7.4-7.5). Sterile-filtered colorless solution.
• Reconstitution: Reconstitute in sterile distilled H2O to no less than 100 μg/mL; dilute reconstituted stock further in other aqueous solutions if needed. Please review COA for lot-specific instructions. Final measurements should be determined by the end-user for optimal performance.

Gene Information

• Gene Name: IL1R2 interleukin 1 receptor, type II [ Homo sapiens (human) ]
• Official Symbol: IL1R2
• Synonyms: IL1R2; interleukin 1 receptor, type II; IL1RB; interleukin-1 receptor type 2; CD121b; CDw121b; IL-1R-2; IL-1RT2; IL-1RT-2; IL-1R-beta; antigen CDw121b; IL-1 type II receptor; interleukin-1 receptor beta; interleukin-1 receptor type II; CD121 antigen-like family member B; type II interleukin-1 receptor, beta; MGC47725
• Gene ID: 7850
• mRNA Refseq: NM_004633
• Protein Refseq: NP_004624
• MIM: 147811
• UniProt ID: P27930

Reviews

10/10/2022

High specificity.

09/12/2020

Good for flow cytometry.

09/20/2016

Suitable for co-immunoprecipitation.

Q&As

What are the functional implications of IL1R2's intracellular domain, and how can engineered receptor constructs shed light on its contributions?

The intracellular domain of IL1R2 has functional significance; engineered constructs clarify its role.

How does IL1R2's glycosylation affect its interaction with IL-1 ligands, and how can glycoproteomics methods elucidate these glycosylation sites?

Glycosylation of IL1R2 affects its IL-1 ligand interaction, and glycoproteomics methods can reveal critical glycosylation sites.

In what ways can engineered IL1R2 variants with altered domains provide insights into its molecular mechanisms and potential therapeutic applications?

Engineered IL1R2 variants offer insights into its mechanisms and therapeutic potential.

In what ways does IL1R2's presence influence the downstream signaling of IL-1 ligands through its competition with IL1R1, and how can genetic manipulation techniques help unravel this interplay?

IL1R2's presence impacts IL-1 ligand signaling through competition with IL1R1; genetic manipulations unveil this interaction.

How do post-translational modifications, particularly ubiquitination, influence IL1R2's stability and turnover?

Post-translational modifications, including ubiquitination, influence IL1R2's stability.

How does IL1R2's expression respond to inflammatory cues, and what role do transcription factors play in its regulatory network?

IL1R2 expression responds to inflammation; transcription factors play a role in its regulation.

What insights can super-resolution microscopy offer regarding IL1R2's intracellular trafficking and subcellular localization dynamics?

Super-resolution microscopy helps understand IL1R2's trafficking and subcellular dynamics in detail.

What specific structural features of IL1R2 enable it to function as a decoy receptor, and how can X-ray crystallography provide a detailed understanding of its ligand binding mechanisms?

Specific structural elements of IL1R2 contribute to its decoy role; X-ray crystallography provides insights into ligand binding.

How does the shedding of IL1R2's ectodomain contribute to its role as a soluble decoy receptor, and which enzymes are involved in this process?

Ectodomain shedding contributes to IL1R2's soluble decoy role; specific enzymes are involved.

What significance does IL1R2's interaction with IL-1 receptor accessory proteins hold in terms of its decoy function?

IL1R2's interaction with accessory proteins is significant for its decoy function.